Associate Professor Ian Cockburn

Group Leader

2014-2018: Australian Research Council discovery grant (DP150102883): To investigate the roles of a novel family of transporters in Apicomplexan biology ($560,000)

2014-2015: Perpetual Foundation (FR2014/1152): Testing of a novel attenuated parasite vaccine ($200,000)

2014: Ian Potter Foundation (32616):  To support the purchase of a multiphoton microscope for intravital imaging studies ($50,000)

Research interests

My group looks at different aspects of the immune response to the malaria parasite Plasmodium. We study not only how CD8+ T cells and antibodies can provide protection, but also how in some cases immune responses can cause pathogenic responses such as cerebral malaria. Your project in the lab would be to use knockout and mutant mice to unravel how these pathogenic responses develop in malaria and, in the longer run, to understand how such responses can be prevented with drugs and therapeutic vaccines.

Chatterjee D, Lewis F, Sutton H, Kaczmarski J, Gao X, Cai Y, McNamara H, Jackson C, Cockburn I (2021), ‘Avid binding by B cells to the Plasmodium circumsporozoite protein repeat suppresses responses to protective subdominant epitopes’ Cell Reports, Volume 35, Issue 2, 108996

Boast B, Miosge L, Kuehn HS, Cho V, Athanasopoulos V, McNamara H, Sontani Y, Mei Y, Howard D, Sutton H, Omari SA, Yu Z, Nasreen M, Andrews TD, Cockburn IA, Goodnow C, Rosenzweig S, Enders A (2021), ‘A Point Mutation in IKAROS ZF1 Causes a B Cell Deficiency in Mice’ The Journal of Immunology April 1, 2021, 206 (7) 1505-1514

Chatterjee D and Cockburn IA (2021) The challenges of a circumsporozoite protein-based malaria vaccine, Expert Review of Vaccines, 20:2, 113-125

Sutton H, Aye R, Idris AH, Vistein R, Nduati E, Kai O, Mwacharo J, Li X, Gao X, Andrews TD, Koutsakos M, Nguyen THO, Nekrasov M, Milburn P, Eltahla A, Berry A, Natasha KC, Chakravarty S, Sim BKL, Wheatley AK, Kent SJ, Hoffman SL, Lyke KE, Bejon P, Luciani F, Kedzierska K, Seder RA, Ndungu FM, Cockburn IA (2021) ‘Atypical B cells are part of an alternative lineage of B cells that participates in responses to vaccination and infection in humans’ Cell Reports, Volume 34, Issue 6, 108684.

Hicks, S.M. and Pohl, K. and Neeman, T. and McNamara, H.A. and Parsons, K.M. and He, J.-S. and Ali, S.A. and Nazir, S. and Rowntree, L.C. and Nguyen, T.H.O. and Kedzierska, K. and Doolan, D.L. and Vinuesa, C.G. and Cook, M.C. and Coatsworth, N. and Myles, P.S. and Kurth, F. and Sander, L.E. and Mann, G.J. and Gruen, R.L. and George, A.J. and Gardiner, E.E. and Cockburn, I.A. 2021 ‘A Dual-Antigen Enzyme-Linked Immunosorbent Assay Allows the Assessment of Severe Acute Respiratory Syndrome Coronavirus 2 Antibody Seroprevalence in a Low-Transmission Setting’ The Journal of infectious diseases, vol. 223, no. 1, pp.10-14.

Coatsworth, N., Myles, P.S., Mann, G.J., Cockburn, I.A., Forbes, A.B., Gardiner, E.E., Lum, G., Cheng, A.C., Gruen, R.L. 2021 ‘Prevalence of asymptomatic SARS-CoV-2 infection in elective surgical patients in Australia: a prospective surveillance study’ ANZ Journal of Surgery

Valencia-Hernandez, A.M., Ng, W.Y., Ghazanfari, N., Ghilas, S., de Menezes, M.N., Holz, L.E. ,Huang, C., English, K. , Naung, M., Tan, P.S., Tullett, K.M., Steiner, T.M., Enders, M.H., Beattie, L., Chua, Y.C. ,Jones, C.M., Cozijnsen, A., Mollard, V., Cai, Y., Bowen, D.G., Purcell, A.W., La Gruta, N.L., Villadangos, J.A., de Koning-Ward, T., Barry, A.E., Barchet, W., Cockburn, I.A., McFadden, G.I., Gras, S., Lahoud, M.H., Bertolino, P., Schittenhelm, R.B., Caminschi, I., Heath, W.R. and Fernandez-Ruiz, D. (2020) A Natural Peptide Antigen within the Plasmodium Ribosomal Protein RPL6 Confers Liver TRM Cell-Mediated Immunity against Malaria in Mice, Cell Host and Microbe, vol. 27, no.6, pp. 950-962.e7

Wang, L.T. and Pereira, L.S. and Flores-Garcia, Y. and O'Connor, J. and Flynn, B.J. and Schön, A. and Hurlburt, N.K. and Dillon, M. and Yang, A.S.P. and Fabra-García, A. and Idris, A.H. and Mayer, B.T. and Gerber, M.W. and Gottardo, R. and Mason, R.D. and Cavett, N. and Ballard, R.B. and Kisalu, N.K. and Molina-Cruz, A. and Nelson, J. and Vistein, R. and Barillas-Mury, C. and Amino, R. and Baker, D. and King, N.P. and Sauerwein, R.W. and Pancera, M. and Cockburn, I.A. and Zavala, F. and Francica, J.R. and Seder, R.A. 2020 ‘A Potent Anti-Malarial Human Monoclonal Antibody Targets Circumsporozoite Protein Minor Repeats and Neutralizes Sporozoites in the Liver’ Immunity, vol. 53, no. 4, pp. 733-744.e8.

McNamara, H.A. and Idris, A.H. and Sutton, H.J. and Vistein, R. and Flynn, B.J. and Cai, Y. and Wiehe, K. and Lyke, K.E. and Chatterjee, D. and KC, N. and Chakravarty, S. and Lee Sim, B.K. and Hoffman, S.L. and Bonsignori, M. and Seder, R.A. and Cockburn, I.A. 2020 ‘Antibody Feedback Limits the Expansion of B Cell Responses to Malaria Vaccination but Drives Diversification of the Humoral Response’ Cell Host and Microbe, vol. 28, no. 4, pp. 572-585.e7

Aye, R. and Sutton, H.J. and Nduati, E.W. and Kai, O. and Mwacharo, J. and Musyoki, J. and Otieno, E. and Wambua, J. and Bejon, P. and Cockburn, I.A. and Ndungu, F.M. 2020 ‘Malaria exposure drives both cognate and bystander human B cells to adopt an atypical phenotype’ European Journal of Immunology, vol. 20, no.8, pp. 1187-1194.

Kelemen, R.K. and Rajakaruna, H. and Cockburn, I.A. and Ganusov, V.V. 2019 ‘Clustering of Activated CD8 T Cells Around Malaria-Infected Hepatocytes Is Rapid and Is Driven by Antigen-Specific Cells’ Frontiers in Immunology, vol.10.

Prabhu, S, Cockburn, I & Garcia de Vinuesa, C (2018) 'HIV Immunogens: Affinity Is Key', Immunity, vol. 48, no. 1, pp. 11-13pp.

Cockburn, I & Seder, R (2018) 'Malaria prevention: from immunological concepts to effective vaccines and protective antibodies', Nature Immunology, vol. 19, pp. 1199-1211pp.

Rajendran, E. and Hapuarachchi, S.V. and Miller, C.M. and Fairweather, S.J. and Cai, Y. and Smith, N.C. and Cockburn, I.A. and Broër, S. and Kirk, K. and Van Dooren, G.G. 2017 ‘Cationic amino acid transporters play key roles in the survival and transmission of apicomplexan parasites’ Nature Communications, vol. 8.

Li, Y.X. and Gautam, V. and Brüstle, A. and Cockburn, I.A. and Daria, V.R. and Gillespie, C. and Gaus, K. and Alt, C. and Lee, W.M. (2017) 'Flexible polygon-mirror based laser scanning microscope platform for multiphoton in-vivo imaging', Journal of Biophotonics, vol. 10, no. 11, pp. 1526-1537.

Papa, I. and Saliba, D. and Ponzoni, M. and Bustamante, S. and Canete, P.F. and Gonzalez-Figueroa, P. and McNamara, H.A. and Valvo, S. and Grimbaldeston, M. and Sweet, R.A. and Vohra, H. and Cockburn, I.A. and Meyer-Hermann, M. and Dustin, M.L. and Doglioni, C. and Vinuesa, C.G. (2017) 'TFH-derived dopamine accelerates productive synapses in germinal centres', Nature, vol. 547, no. 7663, pp. 318+.

Fisher, C.R. and Sutton, H.J. and Kaczmarski, J.A. and McNamara, H.A. and Clifton, B. and Mitchell, J. and Cai, Y. and Dups, J.N. and D'Arcy, N.J. and Singh, M. and Chuah, A. and Peat, T.S. and Jackson, C.J. and Cockburn, I.A. (2017) 'T-dependent B cell responses to Plasmodium induce antibodies that form a high-avidity multivalent complex with the circumsporozoite protein', PLoS Pathogens, vol. 13, no. 7, pp. 23pp.

Sutton, H & Cockburn, I 2017, 'Embracing diversity gives antibodies the power to bind', Immunology and Cell Biology, vol. 95, no. 10, pp. 862-863pp.

McNamara, H, Cai, Y, Wagle, M, Sontani, I, Roots, CM, Misoge, LA, O'Connor, JH, Sutton, HJ, Ganusov, VV, Heath, WR, Bertolino, P, Goodnow, CC, Parish, IA, Enders, A and Cockburn IA. (2017) 'Up-regulation of LFA-1 allows liver-resident memory T cells to patrol and remain in the hepatic sinusoids', Science Immunology, vol. 2, no. 9, pp. 1-10pp.

Li, Y. and Brustle, A. and Gautam, V. and Cockburn, I. and Gillespie, C. and Gaus, K. and Lee, W.M (2016) 'High speed multiphoton imaging', SPIE Biophotonics Australasia, ed. M R Hutchinson and E M Goldys, SPIE - The International Society for Optical Engineering, Bellingham, United States, pp. 5pp.

Cockburn IA and Zavala F (2016) Dendritic cell function and antigen presentation in malaria. Curr Opin Immunol. 40:1-6.

*Radtke AJ, Kastenmüller W, Espinosa DA, Gerner MY, Tse SW, Sinnis P, Germain RN, Zavala FP and Cockburn IA (2015) Lymph-node resident CD8α+ dendritic cells capture antigens from migratory malaria sporozoites and induce CD8+ T cell responses. PLoS Pathog. 6;11(2):e1004637.

Bijker EM, Nganou-Makamdop K, van Gemert GJ, Zavala F, Cockburn I and Sauerwein RW (2015) Studying the effect of chloroquine on sporozoite-induced protection and immune responses in Plasmodium berghei malaria. Malar J. 26;14:130.

Dups JN, Pepper M and Cockburn IA. (2014) Antibody and B cell responses to Plasmodium sporozoites.Front Microbiol. 18;5:625.

Kelemen RK, He GF, Woo HL, Lane T, Rempe C, Wang J, Cockburn IA, Amino R, Ganusov VV, and Berry MW (2014) Classification of T cell movement tracks allows for prediction of cell function. Int J Comput Biol Drug Des. 7(2-3):113-29.

Tse SW, Radtke AJ, Espinosa DA, Cockburn IA and Zavala F (2014) The chemokine receptor CXCR6 is required for the maintenance of liver memory CD8 T cells specific for infectious pathogens. J Infect Dis. 210(9):1508-16.

*Cockburn IA, Tse SW and Zavala F (2014) CD8+ T cells eliminate liver-stage Plasmodium berghei parasites without detectable bystander effect. Infect Immun. 82(4):1460-4.

Ménard R, Tavares J, Cockburn I, Markus M, Zavala F, and Amino R (2013) Looking under the skin: the first steps in malarial infection and immunity. Nat Rev Microbiol. 11(10):701-12.

*Cockburn IA, Amino R, Kelemen RK, Kuo SC, Tse SW, Radtke A, Mac-Daniel L, Ganusov VV, Zavala F and Ménard R (2013) In vivo imaging of CD8+ T cell-mdeiated elimination of malaria liver stages. Proc Natl Acad Sci USA; 110 (22): 9090-9095

Tse SW, Cockburn IA, Zhang H, Scott AL and Zavala F (2013) Unique transcriptional profile of liver-resident memory CD8+ T cells induced by immunization with malaria sporozoites Genes Immun; doi: 10.1038/gene.2013.20

Cockburn IA (2012) Chimeric parasites as tools to study Plasmodium immunology and assess malaria vaccines in “Malaria: Methods and Protocols, 2nd Edition” ed. Robert Menard

*Cockburn IA, Tse SW, Srinivasan P, Radtke A, Chen YC, Sinnis P and Zavala F (2011) Dendritic cells and hepatocytes use distinct pathways to process protective antigen from Plasmodium in vivo. PLoS Pathog; 7 (3): e1001318

Overstreet MG, Chen YC, Cockburn IA, Tse SW and Zavala F (2011) CD4 T cells modulate expansion and survival but not functional properties of effector and memory CD8 T cells induced by malaria sporozoites. PLoS One; 6 (1): e15948

*Cockburn IA, Chen YC, Overstreet MG, Lees JR, van Rooijen N, Farber DL and Zavala F (2010) Prolonged antigen presentation is required for optimal CD8+ T cell responses against malaria liver stage parasites. PLoS Pathog; 6 (5): e1000877

Overstreet MG, Freyberger H, Cockburn IA, Chen YC, Tse S and Zavala F (2010) CpG-enhanced CD8+ T cell responses to peptide immunization are severely inhibited by B cells. Eur J Immunol; 40 (1): 124-133

Joshi T, Rodriguez S, Perovic V, Cockburn IA and Stäger S (2009) B7-H1 blockade increases survival of dysfunctional CD8(+) T cells and confers protection against Leishmania donovani infections. PLoS Pathog; 5(5): e1000431

Srivastava K, Cockburn IA, Swaim A, Thompson LE, Tripathi A, Fletcher CA, Shirk EM, Sun H, Kowalska A, Fox-Talbot K, Sullivan D, Lowenstein CJ, Zavala F and Morrell CN (2008) Platelet Factor 4 mediates inflammation in cerebral malaria. Cell Host Microbe; 4 (2): 179-87

Overstreet MG, Cockburn IA, Chen YC and Zavala F (2008) Protective CD8+ T cells against malaria liver stages: immunobiology of an unnatural immune response. Immunol Rev; 225: 272-83

*Cockburn IA, Chakravarty S, Overstreet MG, Garcia Sastre A and Zavala F (2008) Memory CD8+ T cell responses expand when antigen presentation overcomes T cell self-regulation. J Immunol; 180 (1): 64-71

*Chakravarty S, Cockburn IA, Kuk S, Overstreet MG, Sacci J and Zavala F (2007) Protective CD8+ T lymphocytes against malaria liver stages are primed in skin-draining lymph nodes. Nat Med; 13 (9): 1035-41

Cockburn IA and Zavala F (2007) T cell memory in malaria. Curr Op Immunol; 19 (4): 424-9

*Kaestli M, Cockburn IA, Cortes A, Baea K, Rowe JA and Beck HP (2006) Virulence of malaria is associated with differential expression of Plasmodium falciparum var gene subgroups in a case-control study. J Infect Dis; 193(11):1567-74

Morrot A, Cockburn IA, Overstreet M, Rodriguez D and Zavala F (2006) Protective CD8+ T cells induced by malaria sporozoites do not undergo modulation of interleukin-7 receptor expression. Infect Immun; 74(4):2495-7

Hafalla JC, Cockburn IA and Zavala F  (2006) Protective and pathogenic roles of CD8+ T cells during malaria infection. Parasite Immunol; 28(1-2):15-24

Cockburn IA and Rowe JA (2006) Erythrocyte complement receptor 1 (CR1) expression level is not associated with polymorphisms in the promoter or 3' untranslated regions of the CR1 gene. Int J Immunogenet; 33(1):17-20

Morrot A, Hafalla JC, Cockburn IA, Carvalho LH and Zavala F (2005) IL-4 receptor expression on CD8+ T cells is required for the development of protective memory responses against liver stages of malaria parasites. J Exp Med; 202(4):551-60

*Cockburn IA, Mackinnon MJ, O'Donnell A, Allen SJ, Moulds JM, Baisor M, Bockarie M, Reeder JC and Rowe JA (2004) A human complement receptor 1 polymorphism that reduces Plasmodium falciparum rosetting confers protection against severe malaria. Proc Natl Acad Sci U S A; 101(1):272-7

Cockburn IA, Donvito B, Cohen JH and Rowe JA  (2002) A simple method for accurate quantification of complement receptor 1 on erythrocytes preserved by fixing or freezing. J Immunol Methods; 271(1-2):59-64.