Sanger Sequencing
We are committed to provide an excellent service, accurate results, fast turnaround times, at low cost.
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Services
Sequencing
- Customer completed reactions (user prep)
- Sequencing reactions performed by BRF (core prep)
Genotyping
- Fragment analysis
Tip
The quality of the data is determined by the quality and quantity of the DNA template and primer that you use. Sample cleanup is critical as large excess Big Dye blobs will saturate the signal and salt will prevent correct electrokinetic injection.
Resources
Cost
Price for ANU clients
Price is calculated and quoted when you order via dnaLIMS.
Price for external clients
| Service | External organisation (non-profit) |
|---|---|
| User preps (1-95 samples) | $4.10 per sample |
| User preps (96+ samples) | $2.60 per sample |
| Core preps | $7.20 per sample |
| Fragment analysis | Please contact BRF |
How to order
1. Complete our electronic submission form in dnaLIMS
At the BRF, we use the software dnaLIMS to track and process sanger orders and projects. dnaLIMS is a limited-access online software. ANU customers can use the dedicated sample submission computer located in the Sample Drop Off room (2.043). Customers from CSIRO and UC can access dnaLIMS on computers located at their institutions.
After placing an order, the system generates an order form. Please save an electronic copy and email it to dnaseq@anu.edu.au.
2. Drop off samples
ANU clients with access to JCSMR can drop off their samples in the freezer located at the Sample Drop Off room (2.043).
Clients without access to JCSMR can drop off their samples the freezer located at JCSMR stores at the back of the building. This is available between 8:30am to 12:30pm and 1:30pm to 4:00pm.
If you are posting samples, please use Express Post bags and wrap samples in bubble wrap to protect tubes. Please address your package to:
The Biomolecular Resource Facility (BRF)
The John Curtin School of Medical Research
Building 131
Floor Dock off Garran Road
The Australian National University
Canberra ACT 2601
3. Get your results
ANU customers will receive sequencing files (.ab1 and .seq) via email. Please make sure to update your email in dnaLIMS as your result will be sent there. CSIRO and UC customers can download results by login onto their dnaLIMS accounts.