Sanger Sequencing - core prep samples
Service
Services
The BRF will perform the reactions for you if you elect to use the "Core Prep" service.
Please provide templates and primers at the required concentration and volume in 200µL tubes. Dilute DNA and primers in water.
Recommended stock concentrations
| PCR product <1kB | PCR product >2 kB and ds plasmid | Minimum volume | |
|---|---|---|---|
| Template | 20-50 ng/µL | 100 ng/µL | 15 µL |
| Primer | 1.6 pmol/µL | 1.6 pmol/µL | 15 µL |
If you sequence one template with, for example, three primers, please submit three times as much of the template (ie, 45 µL) in one tube. Similarly, if one primer is to be used on three templates, submit three times as much of that primer (ie, 45µL) in one tube.
We have the following primers available at no extra charge:
- Universal M13 Forward: GTAAAACGACGGCCAG
- Universal M13 Reverse: CAGGAAACAGCTATGAC
- T7: TAATACGACTCACTATAGGG
- Sp6: ATTTAGGTGACACTATAG
- T3: GCAATTAACCCTCACTAAAGG