BA In tissue biology, communities of cells interact with the local environment to form whole tissues that then join to become entire organs. Adherent and differentiated cells exist in a tissue matrix that supports their survival and proliferation are constantly being exposed to different environmental cues. ​Here, we aim to investigate long range cell-cell communication that leads to directional cell migration and formation of tissue units. Our goal is to study how dynamics extracellular conditions, adhesion, fluid flow and microstructure control and regular direct cell-cell communications.