The Lee Group - Optical Biofluidic Imaging Group
Discovering biological physics through optics, computing, materials and fluid dynamics.
Research themes
About
Our team is focus on discovering new biophysical properties of cells and tissue through novel tool building. We have a growing interest in mechanobiology, particularly in the dynamics of tissue formation.
Over the past decade, our team has focused on developing innovative live cell imaging and microfluidic tools; called Spatial Adaptive Imaging and Lithography (SAIL). These tools are designed to quantitatively analyze cell-cell and cell-substrate interactions under various extracellular stimuli, including fluid forces, surface adhesion factors, and 3D topologies. Our research has revealed key biophysical mechanisms that drive cell adhesion and aggregation under fluid pressure. Moving forward, we aim to expand the application of our tools and techniques to engineer biological tissue substitutes on a larger scale.
Cross-disciplinary tool building (Optics, Biofluidic, Computing) that has resulted several industry-ready platform technologies that are being commercialised by Ability Optics Pty Ltd. Currently, we are developing integrated imaging bioreactors that can monitor and produce 3D micro-Avatars of Tissues (MAT) at scale.
Other interests include developing optical tweezers for single-molecule force spectroscopy and pioneering new methods in volumetric imaging from molecular to whole animals scales.
Find out more about the Optical Biofluidic Imaging Group (OBIG) group on their website.
Google Scholar link - Woei Ming Steve Lee
Publications
Selected publications
- Michelle M McDonald...Paul Timpson, Woei Ming Lee.. Peter I Croucher, Tri Giang Phan et al Cell "Osteoclasts recycle via osteomorphs during RANKL-stimulated bone resorption"
- Jun Ki Kim, Woei Ming Lee et al Nature Protocols Fabrication and operation of GRIN probes for in vivo fluorescence cellular imaging of internal organs in small animals"
- Yujie Zheng, Yean Jin Lim, Hanqi Lin, Woei Ming Lee, ACS Photonics "Combined Scattering, Interferometric, and Fluorescence Oblique Illumination for Live Cell Nanoscale Imaging"
- Tienan Xu, Hanqi Lin, Yean J. Lim,.... Woei Ming Lee,. APL Photonics "Computational single-objective scanning light sheet (cSOLS)"
- Yujie Zheng, Samantha J. Montague, Yean J. Lim,.... Woei Ming Lee, Biophys J. "Label-free multimodal quantitative imaging flow assay for intrathrombus formation in vitro"
- Tienan Xu, Yean Jin Lim, Yujie Zheng..., Woei Ming Lee, Lab On Chip "Modified inverted selective plane illumination microscopy for sub-micrometer imaging resolution in polydimethylsiloxane soft lithography devices"
- Zhiduo Zhang, Yujie Zheng, Tienan Xu, ...Woei Ming Lee Biomed. Opt. Express "Holo-UNet: hologram-to-hologram neural network restoration for high fidelity low light quantitative phase imaging of live cells"
Projects
Members
Leader
Group Leader
Researcher
Senior Imaging Scientist
Senior Tutor
Visiting Fellow
Visiting Scientist, Ability Optics Pty Ltd
Visiting Scientist
CEO, Ability Optics Pty Ltd
Visitor
Visiting Postdoctoral Fellow
Student
Honours Student
Honours Student
PhD Student
Research Student
PhD Student
News
The Centre for Computational Biomedical Sciences (CCBS) is on a mission to usher in a new era of biomedical discovery through computational innovation.
JCSMR-led project developed by The Lee Group headed by Dr Woei Ming Steve Lee, was one of the 10 selected medical science projects chosen to bridge the “valley of death” by Australian Economic Accelerator (AEA) scheme.
Cells live within incredibly challenging watery environment. Understanding how different groups of cells know where they are and where to go can spark new possibilities in diagnostics and therapeutics development.