Revvity Opera Phenix High-Content Imaging Microscope
The Revvity Opera Phenix High-Content Imaging Microscope is an advanced imaging platform designed for high-throughput phenotypic screening, offering superior sensitivity and resolution for detailed cellular analysis in various research applications.
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About
The Perkin Elmer Opera Phenix is a sophisticated spinning-disc confocal microscope that contains a microplate stage. While it can be used in the same fashion as a standard confocal microscope, it has been designed for high-throughput, phenotypic screening and assays involving complex disease models, such as live cells, primary cells and micro-tissues. The operating software (Harmony) is also used to develop image analysis algorithms which can take the images that you want to analyse and quantify the specific biology of interest across a large image dataset.
Plate Formats
Optimal for 96, 384, 1536 wells (approved optical microplates), glass slides (aqueous mounting media only)
Types of Imaging
The imager can be used in either standard (wide-field) or confocal modes, thus allowing imaging of 3D structures, or colocalisation analyses to be easily performed. The compartment where the imaging is performed in the instrument is a sealed chamber, and thus is capable of imaging both fixed cells and live cells. Live cell imaging can be performed at a temperature range of 37-42°C and with CO2 (concentration between 1% - 10%) if required. If there is a requirement for multiple timepoints for imaging, plate manipulation (e.g. addition of reagent) or multiple plates to be imaged in a run, the Phenix can be used in tandem with the ACTD robotics and other components within the HCS cabinet to which the Phenix is attached (liquid dispenser/plate washers/37°C incubator/plate hotel).
Lasers & Objectives
The Phenix has 5 excitation lasers and a mixture of air and water objectives, which range from 5x to 63x – this information is provided below. The imager is also capable of capturing label-free brightfield imaging and digital phase imaging. The high NA (Numerical Aperture) water immersion objectives can capture more photons and provides high image resolution even in thick samples. The imager does not have oil-based objectives. Also note that our laser configuration allows FRET-based cellular assays to be performed.
Opera Phenix Objective and Laser/Filter Configuration
| Objective Options | Excitation Laser Options | Emission Filter Options |
|---|---|---|
| 5x Air (NA 0.16) | 375 nm | 435-480 nm |
| 10x Air (NA 0.3) | 425 nm | 435-550 nm |
| 20x Air (NA 0.4) | 488 nm | 500-550 nm |
| 40x Air (NA 0.6) | 561 nm | 570-630 nm |
| 20x Water (NA 0.1) | 640 nm | 650-760 nm |
| 63x Water (NA 1.5) |
Cost
Please contact the ACTD to enquire about pricing.
+61 2 6125 4326
actd.brf@anu.edu.au
How to Book
This instrument is operated by ACTD staff only. For use, please contact the ACTD staff for further assistance.