Nanopore Sequencing (ONT)
A certified Oxford Nanopore sequencing facility, delivering sample QC, library preparation and long-read seequencing solutions to the ANU, to Australia, and to overseas projects with MinION and PromethION technology.
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About
Nanopore sequencing developed by Oxford Nanopore Technologies (ONT) uses molecular sensors or nanopores capable of measuring changes in electric current when a single DNA or RNA molecule passes through them. Different sequences going through a nanopore give different electrical signals, producing an electrical trace or ‘squiggle.’ These squiggles can be decoded into basecalls in real-time. Read lengths produced by Nanopore sequencing are only limited by the size of the library, which enables obtaining DNA reads spanning up to 2Mb and full-length transcripts. Nanopore technology can sequence native DNA and RNA molecules, allowing for the detection of base modifications. The PromethION sequencer is the highest throughput Sequencer from ONT. Each PromethION flow cell is advertised to produce up to 180Gb1 of data.
Applications
Sequencing applications supported by the ONT sequencing include:
- Structural variation
- Assembly
- DNA and RNA base modifications
- Full length transcriptome
- Multiplex sequencing
Products
How to order
1. Check that your samples meet submission criteria
- Refer to the appropriate guide for the amount, concentration, size profile and volume required for the service. For example, for gDNA submission, use the gDNA_ONT guide
2. Fill in the sample submission form in IdeaElan
- Log into IdeaElan and ensure you have access to the BRF Shared Equipment and Store Facility
- Locate the ONT_gDNA form under Request Service > Sample Submission form
- Complete the form with your project details. If you are unsure, plese feel free to get in touch via email or phone.
3. Prepare your samples and bring them to the BRF
- Include a printed copy of the electronic submission form with your samples.
- Submit your samples using 1.5mL microfuge tubes, preferably eppendorf lo-bind.
- Label your tubes with the Sample submission ID (e.g ONT_gDNA) and the Request Id (consecutive numbers from 1) from the "Samples" section of the sample submission form.
- Notify us by email before you intend to bring your samples. If you are shipping, please provide us with an approximate delivery date.
4. Verify if you want to proceed with processing or withdraw your submission.
- Upon reception of your samples, we will conduct quality checks and send you the results to review. Based on these, you will be asked if you wish to proceed with or withdraw your project.