We offer genotyping services for various genetic modifications of mouse/rat lines; including SNPs, transgenes, knock-out/knock-in, conditional/flox-models and various CRISPR induced modifications.

Depending on the assays required, samples are processed by either NGS amplicon sequencing, KASP (Kompetitive allele-specific PCR) or standard gel-based PCR.

Our workflow is optimised for mouse genotyping of mouselines housed at the Australian Phenomics Facility (APF), please contact us for the submission and processing of external samples.

For genotyping of other species, please contact us.

Services

Mouse/rat genotyping for mouselines housed at the APF (ANU internal)

Our workflow is optimised for mouse genotyping of mouselines housed at the Australian Phenomics Facility.

• Genotyping samples are collected by the APF Animal Services staff and submitted for processing. The genotyping results will be uploaded to the APF colony management system ‘Rodentity’ for the end-user to view.
  • All genotyping results are checked against the breeding regime, and any inconsistencies are followed up internally.
• Genotyping settings for animals housed at the APF are managed through Rodentity. Please contact APF Animal Services (animal.ops.apf@anu.edu.au) and BRF Genotyping (genotyping.brf@anu.edu.au) for any changes to the mouseline and allele settings.

Standard genotyping using NGS amplicon sequencing, fluorescence- and gel-based PCR methods

We have a repertoire of >300 genotyping assays for the most common laboratory mouse strains readily available.

• Depending on the assay (s) required, samples will be processed using our semi-automated robotic NGS amplicon sequencing platform, fluorescence end-point PCR or standard gel-based PCR.
• Additionally, if provided with the necessary information, we offer design and validation of specific genotyping assays.

Genotyping assay design

Provided with the necessary information, we offer new genotyping assay development for NGS amplicon sequencing, fluorescence- and gel-based PCR, and Sanger sequencing in line with our workflow.

Genotyping via Sanger sequencing (available upon request only)

In conjunction with the BRF Sanger sequencing service, we offer a full genotyping via Sanger service, including amplification, Sanger sequencing, analyses and will provide a full report via email.

Costs (ANU External)

Genotyping assay design

Assay development and validation against provided samples of known genotype: $150

Genotyping

NGS amplicon sequencing, KASP or PCR assay per allele: $7

Genotyping via Sanger sequencing

Two-directional Sanger sequencing including analysis per reaction: $40