HiC
Reveal the 3D architecture of the genome with HiC: an advanced sequencing technique to examine chromatin conformation.
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About
Hi-C is a powerful genomics technique used to study the 3D organization of chromatin, by capturing physical interactions between different regions of the genome that are spatially close, even if they are far apart linearly on the DNA strand. This is done by crosslinking chromatin, digesting the DNA, ligating proximal fragments together and selecting for these ligated fragments. These samples are processed into high-quality Illumina libraries enriched for ligation products formed between spacially associated DNA. Sequencing these libraries provides a genome-wide contact map that reveals how chromatin folds, identifies topologically associating domains (TADs), and uncovers long-range regulatory interactions. These insights are critical for understanding gene regulation, genome stability, and the spatial context of disease-associated mutations.
The table below details the sample requirements for HiC prep by the BRF. For tissue types other than those listed below, please contact us at brf@anu.edu.au to discuss sample requirements.
| Cell Culture | pellet of 1 million cells |
| Plant | 5 g of young seedling leaves |
| Nucleated Blood | 100 μL of non-coagulated whole nucleated blood in EDTA-coated tubes, or whole nucleated blood preserved in ethanol (containing 100 μL of original blood volume) |
| Animal Tissue | ≥ 0.5 cm2, 50 - 200 mg (the approximate size of a lentil). The preferred order of specimens are: heart, liver, muscle. |
| Insects | whole intact insects. For small insects, please provide multiple specimens to ensure enough DNA can be extracted |
| Fungi | 50 - 200 mg of spores or powder. For filamentous fungi, 100 - 1000 mg |
After organising submission of HiC samples, please complete a submission form and send a copy to brf@anu.edu.au