Transposable elements, transposons in short, are parasitic genetic elements present in every eukaryotic genome sequenced to date. Transposons thrive by increasing the copy number of their genome, the act of which threats the fitness of host organism by damaging the host genes or causing DNA damage. Therefore, the host organisms have acquired sophisticated defence mechanisms to silence the expression of transposon genomes. Notable examples include transcriptional repression via Zinc-Finger motif-containing proteins and Piwi-interacting RNA (piRNA)-guided gene silencing.
The host is constantly exposed to the invasion of new transposons, hence the defence pathways are deemed to be highly adaptive, which creates an evolutionary pressure to invent novel gene expression regulations. We are interested in learning gene expression control mechanisms through the lens of host-transposon interaction and how they in turn play roles in animal development. We currently study several post-transcriptional RNA processing mechanisms that we recently identified by combining cutting-edge biochemical approaches and next-generation sequencing techniques in a model organism fruit fly Drosophila melanogaster.
We are recruiting students of all levels PhD/Honours/Masters/PhB. Potential research projects are listed below. Please feel free to contact us by email (firstname.lastname@example.org) or come to our lab (The Genome Science Department, JCSMR) and talk about your interests as well as about our research.
ORCID (Rippei Hayashi): 0000-0002-5848-9019
Piwi is required to limit exhaustion of aging somatic stem cells.
Sousa-Victor P, Ayyaz A, Hayashi R, Qi Y, Madden DT, Lunyak VV and Jasper H
Cell Reports, 2017 Sep 12; 20(11), 2527-2537
Genetic and mechanistic diversity of piRNA 3'end formation in Drosophila.
Hayashi R*, Schnabl J*, Handler D, Mohn F, Ameres L. S and Brennecke J
Nature, 2016 Nov 24; 539(7630), 588-592 (* equal contribution)
The exon junction complex is required for definition and excision of neighboring introns in Drosophila.
Hayashi R*, Handler D*, Ish-Horowicz D and Brennecke J.
Genes Dev, 2014 Aug 15; 28(16):1772-85. (* equal contribution)
A Genetic Screen Based on in Vivo RNA Imaging Reveals Centrosome-Independent Mechanisms for Localizing gurken Transcripts in Drosophila.
Hayashi R, Wainwright SM, Liddell SJ, Pinchin SM, Horswell S and Ish-Horowicz D.
G3 (Bethesda), 2014 Apr 16;4(4):749-60. (selected for G3 Highlight paper in 2014)
Nuclear Respiratory Factor 2β (NRF-2β) recruits NRF-2α to the nucleus by binding to importin-α:β via an unusual monopartite-type nuclear localization signal.
Hayashi R*, Takeuchi N and Ueda T. (*: corresponding author)
J Mol Biol, 2013 Sep 23;425(18):3536-48.
Drosophila patterning is established by differential association of mRNAs with P bodies.
Weil TT, Parton RM, Herpers B, Soetaert J, Veenendaal T, Xanthakis D, Dobbie IM, Halstead JM, Hayashi R, Rabouille C and Davis I.
Nat Cell Biol, 2012 Dec;14(12):1305-13.
Nuclear Respiratory Factor 2 activates transcription of human mitochondrial translation initiation factor 2 gene.
Hayashi R, Ueda T, Farwell MA and Takeuchi N.
Mitochondrion, 2007 May;7(3):195-203.