The Daria Group - Neurophotonics

The field of neurophotonics bridges optical physics and neuroscience, particularly on the creative use of optical technologies to probe brain function. Neurobiologists now rely heavily on neurophotonics, giving them the necessary tools to analyse the dynamics and principles of neural circuitry, especially on techniques to initiate and detect activity in networks of neurons and to selectively excite sub-sets of neurons. Understanding how these networks of neurons in the brain process sensory inputs and shape motor outputs remains one of science’s great challenges.

Our work is a multi-disciplinary collaborative venture between physicists and neuroscientists to make use of novel optical techniques to analyse the brain. We are mainly interested in using a 3D holographic projection of multiple foci from a single laser source. Each focus functions as a virtual electrode and directed into living brain tissue to manipulate and record neuronal signals in three dimensions.

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VR Daria, ML Castañares, HA Bachor, Spatio-temporal temporal parameters for optical probing of neuronal activity, Biophysical Reviews 13, 13-33 (2021).

ML Castañares, HA Bachor, VR Daria, Analyzing branch-specific dendritic spikes using an ultrafast laser scalpel, Frontiers in Physics 8, 542 (2020)

ML Castañares, G Stuart and VR Daria, Dendritic spikes in apical oblique dendrites of cortical layer 5 pyramidal neurons, BioRxiv (2020)

X Lu, V Gautam, D Shishmarev, VR Daria, Sensing refractive-index gradients along dielectric nanopillar metasurfaces, Optics Express 28, 31594-602 (2020)

L Hanlon, et al, Diamond nanopillar arrays for quantum microscopy of neuronal signals, Neurophotonics 7(3), 035002 (2020).

E Kheradpezhouh, JM Choy, E Arabzadeh, VR Daria, Localized two-photon photoswitching of Optovin in rat cortical neurons, Journ of Phys D: Appl Phys 52, 254001 (2019).

MA Go, M Mueller, M Castanares, V Egger, VR Daria, A compact holographic projector module for high-resolution 3D multi-site two-photon photostimulation, PLoS ONE 14, e0210564, 2019.

V Gautam, S Naureen, N Shahid, Q Gao, Y Wang, D Nisbet, C Jagadish, VR Daria, Engineering highly interconnected neuronal networks on nanowire scaffolds, Nanoletters 17, 3369-3375, 2017

E Kheradpezhouh, JM Choy, VR Daria, E Arabzadeh, TRPA1 expression and its functional activation in rodent cortex, Open Biology 7, 160314, 2017

JM Choy, S Sane, WM Lee, C Stricker, HA Bachor, VR Daria, Improving focal photostimulation of cortical neurons with pre-derived wavefront correction, Frontiers in Cellular Neuroscience 11,105, 2017

MA Go and VR Daria, Light-Neuron interactions: key to understanding the brain, J. Opt 19, 23002 (2017)

YX Li, V Gautam, A Brustle, I A Cockburn, VR Daria, C Gillespie, K Gaus, C Alt, and W M Lee, " Flexible polygon-mirror based laser scanning microscope platform for multiphoton in-vivo imaging" Journal of Biophotonics 10, 1526-1537, 2017

ML Castanares, V Gautam, J Drury, H Bachor, VR Daria, Efficient multi-site two-photon functional imaging of neuronal circuits, Biomedical Optics Express 7 (12), 5325-5334 (2016)

MA Go, JMC Choy, A Colibaba, S Redman, HA Bachor, C Stricker and VR Daria, Targeted pruning of a neuron’s dendritic tree via femtosecond laser dendrotomy, Scientific Reports 6, 19078 (2016)

VR Daria and H-A Bachor, Using light to study neuronal function, European Physics Letters 111, 38001 (2015)

V Gautam, J Drury, JMC Choy, C Stricker, H-A Bachor and VR Daria, Improved two-photon imaging of living neurons in brain tissue through temporal gating, Biomedical Optics Express 6, 4027 (2015)

M Zang, MA Go, C Stricker, VR Daria, A Tricoli, Low-cost photo-responsive nanocarrier platform by one-step functionalization of flame-made Titania agglomerates with L-lysine, Journal of Materials Chemistry B 3, 1677 (2015)

MA Taylor, J Janousek, VR Daria, J. Knittel, B. Hage, HA Bachor, WP Bowen, Subdiffraction-limited Quantum Imaging within a living cell, Phys. Rev. X 4, 011017 (2014)

MA Taylor, J Janousek, VR Daria, J. Knittel, B. Hage, HA Bachor, WP Bowen, Biological measurement beyond the quantum limit, Nature Photonics 7, p229 (2013)

M Go, MS To, C Stricker, S Redman, HA Bachor, G Stuart, VR Daria, Four-dimensional multi-site photoloysis of caged neurotransmitters. Frontiers in Cellular Neuroscience 7, p231 (2013)

M Go, C Stricker, S Redman, HA Bachor, VR Daria, Simultaneous multi-site two-photon photostimulation in three dimensions. Journal of Biophotonics 5, 745-753 (2012)

MA Go, PF Ng, HA Bachor, VR Daria, Optimal complex field holographic projection, Opt. Lett. 36, 3073-3076 (2011)

VR Daria, D Palima, G Gluckstad, “Optical twists in phase and amplitude,” Optics Express 19, p476-481 (2011)

VR Daria, MA Go, HA Bachor, “Simultaneous transfer of linear and orbital angular momentum to multiple low-index particles,” J Opt 13, p044004 (2011) (c 11)

MJ Romero, G Bautista, VR Daria and C Saloma, “Laser confocal microscope with wavelet-profiled point spread function,” Optics Comm. 283, p1217-1221 (2010).

VR Daria, C Stricker, R Bowman, S Redman and HA Bachor, “Arbitrary multi-site two-photon excitation in four dimensions,” Appl Phys Lett 95, 093701 (2009)  (c 45)

G Bautista, MJ Romero, G Tapang and VR Daria, “Parallel photopolymerization of microgear patterns,” Optics Communications 282, 3746-3750 (2009) (c 7)

J Ando, G Bautista, N Smith, K Fujita and VR Daria, “Optical trapping and surgery of living yeast cells using a single laser,” Rev of Sci Instr 79, p103705 (2008) (c 21)

MP David, C Lapid and VR Daria, “An efficient visualization tool for the analysis of protein mutation matrices," BMC Bioinformatics 9, p218 (2008)

D Palima and VR Daria, “Holographic projection of arbitrary light patterns with suppressed zero-order beam,” Appl. Opt. 46, 4197-4201 (2007) (c 45)

M Romero and VR Daria, “Modified filter design to optimize the synthetic reference wave in the generalized phase contrast method,” Opt. Comm 280, 237-242 (2007). (c 2)

D Palima and VR Daria, “Effect of spurious diffraction orders in arbitrary multi-foci patterns produced via phase-only holograms,” Appl Opt 45, 6689-6693  (2006) (c 32)

PJ Rodrigo, VR Daria, J Glückstad, “Four-dimensional optical manipulation of colloidal particles," Appl Phys Lett 86, 074103 (2005) (c 131)

N Arneborg, H Siegumfeldt, G Andersen, P Nissen, VR Daria, PJ Rodrigo, J Glückstad, ”Interactive optical trapping shows that confinement is a determinant of growth in a mixed yeast culture,” FEMS Microbiology Letters 245, 155-159 (2005) (c 58)

PJ Rodrigo, VR Daria, J Glückstad, "Dynamically reconfigurable optical lattices," Opt. Express 13, 1384-1394 (2005) (c 34)

PJ Rodrigo, VR Daria, J Glückstad, Real-time three-dimensional optical micromanipulation of multiple particles and living cells. Opt. Lett. 29, 2270-2272 (2004) (c 89)

VR Daria, PJ Rodrigo, J Glückstad, “Dynamic array of dark optical traps,” Appl Phys Lett 84, 323-325 (2004) (c 60)

PJ Rodrigo, VR Daria, and J Glückstad, Real-time interactive optical micromanipulation of a mixture of high-and low-index particles, Opt. Express 12, 1417-1425 (2004) (c 56)

J Glückstad, VR Daria, PJ Rodrigo, Decrypting binary phase patterns by amplitude. Opt. Engineering 43, 2250-2258 (2004)

VR Daria, PJ Rodrigo, S Sinzinger, J Glückstad, Phase-only optical decryption in a planar-integrated micro-optics system, Opt. Engineering 43, 2223-2227 (2004)

VR Daria, PJ Rodrigo, J Glückstad, “Programmable complex field coupling to higher order guided modes of microstructured fibers,” Optics Comm 232, 229-237 (2004)

VR Daria, PJ Rodrigo, J Glückstad, “Dynamic formation of optically trapped microstructure arrays for biosensor applications,” Biosensors and Bioelectronics 19, 1439-1444 (2004)

VR Daria, RL Eriksen, J Glückstad, Dynamic optical manipulation of colloidal structures using a spatial light modulator. Journ of Modern Optics 50, 1601-1614, (2003) (c 13)

J Glückstad, VR Daria, PJ Rodrigo, Comment on "Interferometric phase-only optical encryption system that uses a reference wave". Opt. Lett 28, 1075-1076 (2003)

R Eriksen, VR Daria, PJ Rodrigo, and J Glückstad, Spatial light modulator controlled alignment and spinning of birefringent particles optically trapped in an array. Appl Optics 42, 5107-5111 (2003)

VR Daria, R Eriksen, S Sinzinger and J Glückstad, Optimising the generalised phase contrast method in a planar-optical device. Journ of Optics: Pure and Appl Optics 5, s211-s215 (2003)

RL Eriksen, PJ Rodrigo, VR Daria, J Glückstad, Computer-controlled orientation of multiple optically-trapped microscopic particles. Microelectronic Engineering 67-68, 872-878 (2003)

PJ Rodrigo, RL Eriksen, VR Daria, J Glückstad, Shack-Hartmann multiple-beam optical tweezers. Opt. Express 11, p208. (2003)

J Glückstad, VR Daria, RL Eriksen, Comment: Influence of illuminating beyond the object support on Zernike-type phase contrast filtering. Appl. Opt. 42, p762. (2003)

RL Eriksen, VR Daria, J Glückstad, Fully dynamic multiple-beam optical tweezers. Opt. Express 10, p597. (2002) (c 191)

PJ Rodrigo, RL Eriksen, VR Daria, J Glückstad, Interactive light-driven and parallel manipulation of inhomogeneous particles. Opt. Express 10, p1550. (2002) (c 95)

VR Daria, J Glückstad, P Mogensen, R Eriksen, S Sinzinger, ”Implementing the generalized phase contrast method in planar-integrated micro-optics platform,” Opt Lett 27, p945 (2002)

VR Daria, J Miranda and C Saloma, "High-contrast images of semiconductor sites via one-photon optical beam induced current imaging and confocal reflectance microscopy," Applied Optics 41, p4157 (2002)

VR Daria, Caesar Saloma & S Kawata, "Excitation with a focused, pulsed optical beam in scattering media: reply to comment," Applied Optics 41, p4652 (2002)

VR Daria, C Saloma and S Kawata, “Excitation with a focused, pulsed optical beam in scattering media: diffraction effects,” Applied Optics 39, p5254 (2000)

VR Daria and C Saloma, “High-accuracy fourier transform interferometry without oversampling with a 1-bit analog-to-digital converter,” Applied Optics 39, p108 (2000)

VR Daria, C Blanca, O Nakamura, S Kawata and C Saloma, “Image contrast enhancement for two-photon fluorescence microscopy in turbid medium,” Applied Optics 37, p7960 (1998)

VR Daria, O. Nakamura, C Palmes-Saloma and S Kawata, “Enhanced depth penetration in imaging of turbid biological samples using two-photon fluorescence microscopy,” Jpn. Journ of Appl Phys 37A, p959 (1998)

VR Daria and C Saloma, “Bandwidth and detection limit of a crossing-sampling spectrum analyzer, Review of Scientific Instruments 68, p240, (1997)

C Blanca, VR Daria and C Saloma, "Spectral Recovery by analytic continuation in crossing-based spectrum analysis,” Applied Optics 35, p6417 (1996)

C Saloma, VR Daria and J Munoz, "Fourier transform refractometry using multichannel detection", Applied Optics 32, p4785 (1993)

C Saloma and VR Daria, "Performance of a zero-crossing optical spectrum analyzer", Optics Letters 18, p1468 (1993)


VR Daria, J Miranda and C Saloma, Method for generating high contrast images of semiconductor integrated circuits, US Patent: 7235988; WIPO: WO/2004/008164; IP Philippines: 1-2005-50056.

Edited proceedings

F Pavone, E Hillman, VR Daria and S Charpak, Progress in biomedical optics imaging, Proc. of OSA Biomedical Optics-SPIE Vol 8804, 880401.

V Daria (Editor), Book of Abstracts, Global Nanophotonics 2017

V Daria (Editor), Pisika, Volumes 1 and 2 (4 Issues per volume), 2007-09

V Daria (Editor), Proceedings of the Samahang Pisika ng Pilipinas, 2007-09

Book Chapters

VR Daria and WM Lee (2019) Optical Nanomanipulation and Structured-Beam Optical Traps. In: DL Andrews, RH Lipson and T Nann (eds) Comprehensive Nanoscience and Nanotechnology 2nd Ed, 4, 347–360. Oxford: Academic Press.

M Castanares, G Stuart, VR Daria (2019), Holographic functional imaging of neuronal circuit activity,In  FJ Kao, G Keiser, A Gogoi (eds) Advance Optical methods for Brain Imaging, 5, 143-165, Springer Nature Singapore Pte Ltd.

VR Daria, D Palima and J Glückstad (2012), “Efficient generation of optical twisters using helico-conical beams.” In: The Angular Momentum of Light, Cambridge University Press, 352-364

PJ Rodrigo, VR Daria and J Glückstad. “Four-dimensional optical manipulation of colloidal particles.” In: Optical Tweezers, Methods and Applications (CRC Press, 2010).

S Sinzinger, J Jahns, VR Daria and J Glückstad, "Planar microoptical systems for correlation and security applications." In: Optical imaging sensors and systems for homeland security applications. Javidi, B. (ed.), (Springer, Berlin, 2006) p339-366