Version 6.0, January 2018

Background and purpose

This Client Service Charter communicates to our clients what they can expect of our services and set clear goals for our staff.

This Client Service Charter forms part of the facility’s Quality Management Framework that outlines the responsibilities of staff who deliver our key requirements. Supporting these policies and standards will be Standard Operating Procedures (SOP’s) and Safe Work Procedures (SWP’s) that define how individual procedures and activities should be undertaken.

This document is available to all staff at the APF and clients to ensure they are aware of the full parcel of services and goals of the facility. If you would like to review the facility’s quality documents please contact us.

We seek and welcome your feedback on our service delivery.

Mission

The Australian Phenomics Facility supports open access large-scale phenotyping of humans and mice to uncover the biological drivers in human disease.

Quality policy statement

To provide a quality, best practice, science capability to support Australian biomedical research.

To go the extra distance in delivering efficient services to our researchers, clients, and industry collaborators.

To contribute to managing and sharing information and at the same time protecting privacy and client confidentiality.

Support excellence through training, continual improvement and a dynamic work environment.

Who we are

The Australian Phenomics Facility specialises in creating, characterising and archiving of mouse models of human disease.

Established in 2004, the facility receives funding from the Australian Government’s NCRIS Super Science and previously from CRIS through the Australian Phenomics Network and contributions from the Australian National University. The Animal Services Division receives funding support from JCSMR and the office of the DVCR.

We have an open access policy and support academic and corporate research programmes in Australia and internationally.

The facility is equipped with up to date technology ensuring the highest quality in all aspects of our work.

Our aim is to service research in a time efficient manner with quality performance targets through

• Timely services
• Highly skilled technical staff
• Training and Development
• Consultation and planning

All services will be compliant with relevant legislation including those governed by the Office of the Gene Technology Registrar (OGTR), Animal Experimentation Ethics Committee (AEEC), Department of Agriculture and other relevant legislative authorities.

Communication

We understand that when using our services you may have a range of different enquiries and as such, this can involve coordination of multiple service areas.

To facilitate requests in an efficient and timely manner we provide key contacts for all service areas. This list can be used in conjunction with the APF Contact Sheet.
 

Area	Contact email address
Level 3 Production/Breeding	animal.ops.apf@anu.edu.au
Level 2 Experimental	animal.ops.apf@anu.edu.au
Containment and Behavioural Suites	animal.ops.apf@anu.edu.au
Imports/Exports	animal.shipments@anu.edu.au
Phenome Bank (cryopreservation and reanimation included)	phenbank@anu.edu.au
General Enquiries	administration.heb@anu.edu.au
IT Services (Databases and Rodentity)	it.apf@anu.edu.au
Training	training.apf@anu.edu.au

Although communication by telephone is sometimes necessary, it is our general policy to communicate with our clients in writing, via email whenever possible. This lowers the interruption factor and allows increased staff efficiency. It also provides you with an electronic record of the enquiry and response. Where relevant, APF staff will confirm phone conversations by email.

Technical staff check their emails at least twice daily. However, if you have an urgent enquiry please contact the area coordinator (listed on the APF Contact sheet) who will be able to direct your query appropriately.

For the majority of enquiries you can expect an initial response within 48 hours.

Managers also attend regular committee meetings including the JCSMR Animal Users Committee and ANU Animal Experimentation Ethics Committee. We also hold specific area meetings to deal with day-to-day operational aspects of the facility. We welcome you to provide agenda items to the facility run meetings for discussion at the appropriate forum.

For projects that you have engaged the APF for, we aim to provide written progress reports at key milestones throughout the project.

At the APF, we welcome constructive criticism and feedback about services delivered as well as appreciation and suggestions on how we might improve them. If you have any feedback or complaints about any of our services please contact us administration.heb@anu.edu.au

Service commitments

Animal services

The APF recognises that time delays in providing services can have significant impact on research and cost implications and will therefore strive to meet the following targets for particular services. Where we are unable to meet these goals, the APF will aim to notify researchers in advance and give an expected revised timeframe.

Service	Delivery Timeframe
Breeder Set Up *	Within 10 working days of request
Culling (not managed) *	Within 6 working days of request
Sample Collection (organs etc)	Within 5 working days of request
DNA collection (continual maintenance)	By 28 days of age
DNA collection (special request)	Within 5 working days of task request submission
Phenotyping (special request)	Within 1 week of task request submission
Supply of clean cages and associated goods	Within 3 days of request
Processing of accounts (email out)	Within 10 working days of end of (ANU financial) period
Importation and rederivation of strain *	10 months from date of import
Cryopreservation of mice	Within 12- 16 weeks of request
Export of sperm or live mice	Typically within 3 months subject to MTA and other required approvals

*Contact us for more information on detailed timeframes

Genotyping

A comprehensive service relating to genotyping, including ear punch and tissue digestion through to assay design and validation. The BRF- Genotyping team has an in-depth understanding of mouse genetics and mouse models and designs complex assay for both CRISPR modified mice and conventionally targeted genetically modified mice.

Service Request	Delivery Timeframe
Genotyping (existing assay)	Within 14 days of sample submission
New genotyping assay design (design, order, optimisation)	4 - 6 weeks
DNA preparation (plate- crude)	Within 3 days of sample submission
Sanger sequencing	4 - 6 weeks
Sanger sequencing (existing assay)	Within 3 weeks of sample submission

Training

We have extensive expertise in the area of mouse breeding and research, mouse handling and various specialised techniques.

The APF provides training in a number of skills including basic mouse handling, use of the Musterer database, advanced mouse and rat techniques and anaesthesia.

For comprehensive training services please contact us at training.apf@anu.edu.au

Enrolment in the majority of training courses is available on HORUS.

Courses offered by the APF AS team;

Course Code	Description	Reassessment
ANML02	Introduction to Mouse Handling	3 years (ANML39)
ANML05	Sharps Safety with Animals	3 years (ANML39)
ANML06	Intraperitoneal Injection - Mouse	3 Years
ANML08	Intravenous Injection - Mouse	3 Years
ANML11	Introduction to Rodentity	As Required
ANML20	Retro-Orbital Sinus Blood Collection - Mouse	Yearly
ANML21	Tail Vein Blood Collection - Mouse	3 Years
ANML22	Introduction to Animal Anaesthesia - Mouse	3 Years
ANML33	Oral Gavage - Mouse	Yearly
ANML34	Intramuscular Injections - Mouse	3 Years
ANML35	Subcutaneous Injection - Mouse	3 Years
ANML36	Intradermal Injection - Mouse	3 Years
ANML37	Cardiac Bleed - Mouse	3 Years
ANML38	Intranasal Administration - Mouse	3 Years
ANML39	Mouse Care and Handling Refresher

Service definitions

Clients should be familiar with a variety of terms that the facility uses, below is a list of common definitions used by technicians and managers:

Definitions

Managed Culling – the process by which whole cages or individual animals are nominated for culling by the researcher (Swift Cull) in managed strains. Mice once nominated move to the swift cull list for culling within one week. Mice can be added to the cull list until 5pm Friday. Mice can be saved from culling if accidentally selected until Sunday afternoon.

APF Managed Strain – the researcher requests that the APF takes responsibility for breeding and maintaining the strain based on projections and the stated requirements of the research group. Researchers must provide a maximum cage number they are willing to hold the strain at and decisions can be made in consultation with the research group.

Continual Maintenance – for the purpose of maintaining the genotyping requirements applied to the strain at each generation. i.e a strain on continual maintenance will have all stock punched for genotyping purposes.

Projections – strain and number of animals likely to be needed by a research group, this is requested quarterly to ensure future orders can be fulfilled. Projections allow the APF to manage the number of breeders required etc but do not mean animals are set aside for orders.

Standing Orders – A regular ongoing order for animals on a regular basis both in house and externally. A standing order will mean that as soon as animals are available (often as early as weaning age) they will be assigned to orders, ensuring best availability.

Multiple User Strain – APF owned strain used by multiple research groups i.e. C57BL/6. The APF covers the holding cost of these animals but the animals are charged for as an individual animal purchase price. To classify as a Multiple User Strain the following criteria must be met:

• At least 30% of its use must be shared by more than one user and preferably 3 or more users
• Multiple users must require the strain for a time period greater than 6 months
• There must be more than 10 animals ordered per month

Single User Strain – Strain charges maintained by an individual or group. Permission and approval required for use by all others. Breeding of single user strains may be managed either by the researcher (User Managed) or by the APF Breeding Coordinator (APF Managed).

• Where a strain is held under the APF Breeding Protocol (A2015/08 Fowler or relevant replacement) the breeding must be overseen and approved by the Scientific Services Manager (Stuart Read) or Head of Animal Services (Suzanne Fowler). It is required that the breeding for these strains is APF Managed to ensure the breeding practices in place are in line with the protocol approved by the ANU Animal Experimentation Ethics Committee.
• Where a researcher monitors the breeding and numbers of animals bred on their own protocol, they must take responsibility for the breeding oversight of the strain or provide clear, written guidelines to the Breeding Coordinator and APF management on their breeding requirements. The researcher must complete and provide the APF Strain Information Form, including any changes to requests for strain management.
• An individual who holds and pays for the maintenance of a single user strain may elect to share the strain with collaborators at the ANU or externally. The APF will facilitate transfer of animals subject to written approval from the holder of the strain. Any cost reimbursement is the responsibility of the holder of the strain and will not be managed by the APF.

Short Notice Request – Requests made outside APF specified delivery timeframes.

Ordering and Availability

Multi User Strains

Multiple user strains are kept at the lowest colony size to ensure minimal wastage of animals according to ethical guidelines. Orders for multiple user strains should be placed well in advance and where possible, standing orders should be placed to ensure availability of mice. Larger orders (10 mice or more) may require more than 6 weeks’ notice for some strains that are high demand.

Late Cancellation (less than 48 hours notice) of standing orders will be charged at 50% of the cost.

Mice will not be kept past 6 weeks of age to minimise wastage and ensure efficient colony management. Any animals required beyond this age will be supplied at 6 weeks and the researcher will incur the cage per week cost from that point. Animals can be held in the Hugh Ennor Building until age required.

Externally Sourced Animals

Orders of animals from external suppliers will be charged at cost to the researcher.

Importation of animals into the APF falls into one of two categories:

Approved supplier- this is a commercial vendor overseas (e.g. Jackson Laboratories, Charles River) OR  within Australia (e.g. Australian Bioresources, Ozgene ARC)

Non-approved supplier- all non-commercial animal sources, e.g. universities, research institutes

Animals from overseas whether or not they are from approved or non-approved suppliers are placed into our Quarantine Area on arrival and any required health screening is conducted. Once animals are released from Quarantine, they are housed in designated animal holding room.

Animals imported from overseas or from within Australia to be bred at APF must be rederived. Rederivation is a method by which animals can be “cleaned” or decontaminated of certain pathogens. The process involves transferring cleaned embryos to the reproductive tract of a clean recipient female. Rederivation can be done via in-vitro fertilization (IVF) or via live mating.

If lines imported are frozen embryos or sperm, please submit reanimation request to phenbank@anu.edu.au. Rederivation request can also be placed to this address.

All other imports are subject to approval by the Head of Animal Services.

For further information or questions, please contact Import/Export Coordinator via animal.shipments@anu.edu.au.

Service Inclusions

Cage Holding Services

Husbandry

• Cage husbandry including cage changing performed as required relevant to the caging type, number of animals per cage and health of the animals.
• Cage changing with enriched corn cob bedding (includes nesting material only) or Aspen bedding if requested by research groups
• Additional enrichment options available, ie. tissue, cardboard tunnel, crinkle disc
• Provision of standard irradiated rodent breeding chow, automatic watering (or bottled water, where requested and indicated by the experimental program) and Hydrogel where indicated.
• Daily husbandry and basic welfare check of animals – requests for additional checks may incur a charge as Category B service provision.

Breeding

• Breeder set ups as requested by researchers via Rodentity Database.
• The APF can, where requested, manage breeding to within specifications by a research group.
• Weaning – APF staff will wean pups between 19 and 22 days of age.
• Researchers are ultimately responsible for monitoring of poor breeders unless the strain is an “APF Managed Strain” in which case the APF Breeding Coordinator will oversee these requirements.

Punching

• Ear punching as per client service charter expectations (28 days).

Bleeding

• Bleed requests can be made via Rodentity database. Minimum of 10 working days’ notice is required.

Genotyping

• • Services included are genotyping of mice that are housed at the APF Genotyping of animals with no fixed genotype is included in the cage price for standard PCR or KASP assays. Costs of re-genotyping mice will be at the discretion of the genotyping facility.

Veterinary care

• Veterinary care and monitoring of unwell animals including investigations of unexpected events.
• Routine health monitoring is performed on the majority of animals within the colony.
•  Health reports are available on the website and by contacting the facility. Requests for additional health screening will be charged at cost.

Animal Delivery

• Delivery of animals to other APF managed facilities or the JCSMR Holding Room as requested via the Rodentity database move mouse or order mice request.
• Animals that require picking up at the Hugh Ennor Building (HEB) must be collected within 10 minutes of the arranged pick up time. Animals that have been removed from the HEB barrier cannot be returned and will be euthanised if pick up does not occur in a timely fashion.

Timed Mating

• For mice provided before 14 days gestation – plug guarantee only. Plug indicates a mating has occurred but cannot guarantee pregnancy.
• For mice that are issued post 14 days gestation – we expect a 90% pregnancy rate. Some mice may show signs of abortion.

Cage Supply

• Cages will be supplied with enriched bedding.
• Additional reasonable requests for food and sterilized water can be made without additional charge.
• Satellite Facilities- Cages must be changed by the research group (unless otherwise negotiated) and changed within ethical timeframes and cages must be rotated for washing with each cage change.

Importation, Quarantine and Rederivation

• Importation of animals is charged for as per shipment fees detailed in this document
• Importation will not take place until the client completes the required documentation including strain information, quotes or other relevant forms.
• The APF will assist with the appropriate document requirements, including MTAs and the institutions required to be involved based on the information provided. The Innovations Team must approve changes to MTAs. Delays in this process are not the responsibility of APF staff.
• The standard charge rate applies for cages held in quarantine areas, including Quarantine Approved Arrangement Sites (International Quarantine as approved by Department of Agriculture) areas.
• The client will pay for the cost of health screening for release of animals from quarantine, for the cost of rederivation and health screening post rederivation.
• The client will pay the cost of courier fees and shipping boxes.

All service inclusions are subject to the timeframes and notice periods outlined in the Client Service Charter. Additional requests at short notice may be charged for as Category B services for the time taken to arrange and deliver the requested service.

Record keeping

The APF uses an in-house database known as Rodentity to monitor, track and organise all animal information. This database provides significant information to researchers and APF staff in the management of strains and invoicing details. The Rodentity interface is completely web based and can be accessed remotely when required. Rodentity can be found at: https://rodentity.apf.edu.au/

The Rodentity database provides an efficient interface for researchers within the ANU to request animal and genomics services. The APF has a policy on the retention of data produced by the facility. The APF retain all scientific raw and analysed data in laboratory notebooks and/or electronically by the APF for a minimum of five years from the completion of the task/project with the exception of raw data generated from next generation sequencing services.

The APF has a barcoding and storage system for tracking samples collected and processed by the facility.

Quality

We have a commitment to quality through continuous improvement. As such, we have committed ourselves to the following:

• Training and development for our employees
• Refreshing of mouse colonies to prevent genetic drift
• Review of staff technique to prevent “drift” from the procedure and to maintain quality
• Measurable quality objectives which reflect our service aims
• Efficient and courteous service.

Subcontracting

Work may occasionally be subcontracted to other parties including;

• Projects that require technical expertise or processes not available at the APF
• Large projects that extend beyond the APF’s capacity
• During periods of peak workload
• Due to unforeseen circumstances

In the event that the APF subcontracts any work the client will be notified prior to commencement of the subcontracted work. The APF takes due steps to ensure that subcontractors are competent and of a high quality by reviewing the company’s certificates of accreditation and/or registration, check of sample results or audits where appropriate.

Typical work that may be subcontracted includes;

• Courier services
• Health screening of animals

If a client selects a particular subcontractor, the APF will not bear responsibility for the results.

Privacy

At the APF, we are committed to protecting your privacy.

We have put in place appropriate physical, electronic, and managerial procedures to safeguard and help prevent unauthorised access, maintain data security and ensure the correct use of service information.