Dr Viviane Delghingaro-Augusto

PhD
Research Fellow

I obtained my PhD in Functional and Molecular Biology at University of Campinas, Sao Paulo, Brazil. I received further training as an islet β-cell biologist in the laboratories of Prof Prentki (Post-doctoral Scientist, Montreal, Canada) and Prof Nolan (ANU, Australia). In my first postdoctoral position I performed key experiments in pancreatic islets of several rodent models and pancreatic β-cell lines resulting in the discovery of the islet β-cell glycerolipid/free fatty acid cycle, an essential component of the lipid amplification pathway of glucose-stimulated insulin secretion. This work has resulted in papers in Cell Metabolism, Journal of Clinical Investigation and Diabetologia. The research has led to new targets for drug discovery in treatment for type 2 diabetes (patents active - Prentki laboratory). Since in Canberra in my second post-doctoral position, I continued to develop my skills in pancreatic islet research. I contributed with extensive experimental data to a major publication (equal first author) in Nature Genetic, describing the existence of pancreatic β-cell fragility in T1D and T2D mice models. I also completed extensive experimental work on a NHMRC funded project on the 'Role of islet β-cell failure in the pathogenesis of non-alcoholic steatohepatitis' investigating the role of insulin hypersecretion (manuscript under preparation). My extensive research training provided me with highly specialized skills in islet β-cell research, including in vivo metabolic physiology assessment in rodent models, islet isolation from mice and rats, in vitro insulin secretion studies and assessment of islet metabolism, pancreas and islet histological examination including immunohistochemistry and β-cell mass measurements, and ultra-structural examination by electron microscopy. I have also developed skills in gene expression analysis using various techniques, including high-throughput platforms.

Research interests

My main areas of research are: 1) the identification of metabolic coupling factors involved in glucose-stimulated insulin secretion with a particular interest islet β-cell lipid metabolism and lipid signalling molecules, and 2) the investigation of the molecular and biochemical mechanisms involved in pancreatic islet β-cell adaptation and failure to different stressors in animal models.

  • Delghingaro-Augusto, V, Hosaka, A, Estaphan, S et al. 2023, 'High Dietary Iron in Western Diet-Fed Male Rats Causes Pancreatic Islet Injury and Acute Pancreatitis', Journal of Nutrition, vol. 153, no. 3, pp. 723-732.
  • Kim, J, Delghingaro-Augusto, V, Chang, J et al. 2022, 'The role of fatty acid signaling in islet beta-cell adaptation to normal pregnancy', Frontiers in Endocrinology, vol. 12, pp. 1-15.
  • Lee, K, Chan, Y, Liang, C et al. 2022, 'XBP1 maintains beta cell identity, represses beta-to-alpha cell transdifferentiation and protects against diabetic beta cell failure during metabolic stress in mice', Diabetologia, vol. 2022, no. 65, pp. 984 - 996.
  • Waters, M, Delghingaro-Augusto, V, Javed, K et al. 2021, 'Knockout of the Amino Acid Transporter SLC6A19 and Autoimmune Diabetes Incidence in Female Non-Obese Diabetic (NOD) Mice', Metabolites, vol. 11, no. 665.
  • Dagpo T, Nolan CJ & Delghingaro-Augusto V 2020, 'Exploring Therapeutic Targets to Reverse or Prevent the Transition from Metabolically Healthy to Unhealthy Obesity', Cells, vol. 9, no. 7, pp. 1-23.
  • Nolan PhD, C & Delghingaro-Augusto, V 2016, 'Reversibility of Defects in Proinsulin Processing and Islet beta-Cell Failure in Obesity-Related Type 2 Diabetes', Diabetes, vol. 65, no. 2, pp. 352-354.
  • Dooley, J, Tian, L, Schonefeldt, S, Delghingaro-Auugsto V, et al. 2016, 'Genetic predisposition for beta cell fragility underlies type 1 and type 2 diabetes', Nature Genetics, vol. 48, no. 5, pp. 519-527.
  • Zhao, S, Mugabo, Y, Iglesias, J et al 2014, 'alpha/beta-Hydrolase Domain-6-Accessible Monoacylglycerol Controls Glucose-Stimulated Insulin Secretion', Cell Metabolism, vol. 19, no. 6, pp. 993-1007.
  • Delghingaro-Augusto, V, Madad, L, Chandra, A et al. 2014, 'Islet inflammation, hemosiderosis, and fibrosis in intrauterine growth-restricted and high fat-fed sprague-dawley rats', American Journal of Pathology, vol. 184, no. 5, pp. 1446-1457.
  • Farrell, G, Mridha, A, Yeh, M et al. 2014, 'Strain dependence of diet-induced NASH and liver fibrosis in obese mice is linked to diabetes and inflammatory phenotype', Liver International, vol. 34, no. 7, pp. 1084-1093.
  • Nolan, C & Delghingaro-Augusto, V 2014, 'RNA sequencing of all transcripts and how islet beta-cells fail', Diabetes, vol. 63, no. 6, pp. 1823-1825.
  • Cheng, K, Delghingaro-Augusto, V, Nolan PhD, C et al. 2012, 'High passage MIN6 cells have impaired insulin secretion with impaired glucose and lipid oxidation', PLOS ONE (Public Library of Science), vol. 7, no. 7.
  • Delghingaro-Augusto, V, Décary, S, Peyot, M et al 2012. Voluntary running exercise prevents beta-cell failure in susceptible islets of the Zucker diabetic fatty rat, American Journal of Physiology - Endocrinology and Metabolism, vol. 302, no. 2, pp. E254-E264.
  • Tiano JP, Delghingaro-Augusto V, Le May C et al. 2011 Estrogen receptor activation reduces lipid synthesis in pancreatic islets and prevents beta cell failure in rodent models of type 2 diabetes Journal Clinical Investigation, vol. 121(8):3331-42
  • Pathmaperuma A, Mana P, Cheung SN, Kugathas K, Koina ME, Delghingaro-Augusto V, Ellwood DA, Dahlstrom JE, Nolan CJ 2010. Fatty acids upregulate their own storage into lipid droplets and promote cell aggregation and cytokine production independent of glucose in human cytotrophoblasts. Placenta, vol 31(3):230-9.
  • Pepin E, Guay C, Delghingaro-Augusto V et al. 2010 Short-chain 3-hydroxyacyl-CoA dehydrogenase is a negative regulator of insulin secretion in response to fuel and non-fuel stimuli in INS 832/13 β-cells. Journal of Diabetes, vol. 2(3):157-67.
  • Delghingaro-Augusto V, Nolan CJ, Gupta D, et al. 2009. Failure of b-cell compensation in the 60% pancreatectomized obese Zucker fatty rat. Severe dysfunction without steatosis or a further decreased β-cell mass. Diabetologia, vol. 52(6): 1122-32.
  • Lamontagne J, Masiello P, Marcil M, Delghingaro-Augusto V, Burelle Y, Prentki M, Nolan C. 2007 Circulating lipids are lowered but pancreatic islet lipid metabolism and insulin secretion are unaltered in exercise-trained female rats. Applied Physiology Nutrition and Metabolism, vol.32(2):241-248.
  • Nolan C., Madiraju MS, Delghingaro-Augusto V et al. 2006 Fatty acid signaling in the β-cell and insulin secretion. Diabetes, vol. 55 Suppl 2:S16-23.
  • Nolan CJ, Leahy JL, Delghingaro-Augusto V et al. 2006 Beta cell compensation for insulin resistance in Zucker fatty rats: increased lipolysis and fatty acid signaling 2006. Diabetologia, vol 49(9): 2120-2130. Diabetologia, vol(9): 2120-2130
  • Gremlich S, Nolan C, Roduit R, Burcelin R, Peyot M-L, Delghingaro-Augusto V, Desvergne B, Michalik L, Prentki M, Wahli W 2005. Pancreatic islet adaptation to fasting is dependent on peroxisome proliferator-activated receptor alpha transcriptional up-regulation of fatty acid oxidation. Endocrinology, vol. 146(1):375-82.
  • Araujo EP, Amaral ME, Filiputti E, De Souza CT, Laurito TL, Augusto VD, Saad MJ, Boschero AC, Velloso LA, Carneiro EM 2004. Restoration of insulin secretion in pancreatic islets of protein-deficient rats by reduced expression of insulin receptor substrate (IRS)-1 and IRS-2. J Endocrinol.vol. 181(1):25-38
  • Bordin S, Amaral ME, Anhe GF, Delghingaro-Augusto V, Cunha DA, Nicoletti-Carvalho JE, Boschero AC 2004. Prolactin-modulated gene expression profiles in pancreatic islets from adult female rats 2004. Mol Cell Endocrinol. vol.31; 220(1-2):41-50.
  • Delghingaro-Augusto V, Ferreira F, Bordin S et al. 2004 A low protein diet alters gene expression in rat pancreatic islets. J Nutr. vol.134(2):321-7.
  • Ferreira F, Barbosa HC, Stoppiglia LF, Delghingaro-Augusto V, Pereira EA, Boschero AC, Carneiro EM 2004. Decreased insulin secretion in islets from rats fed a low protein diet is associated with a reduced PKA alpha expression. J Nutr. vol. 134(1):63-7
  • Roduit R, Nolan C, Alarcon C, Moore P, Barbeau A, Delghingaro-Augusto V, Przybykowski E, Morin J, Masse F, Massie B, Ruderman N, Rhodes C, Poitout V, Prentki M 2004. A role for the malonyl-CoA/long-chain acyl-CoA pathway of lipid signaling in the regulation of insulin secretion in response to both fuel and nonfuel stimuli. Diabetes. vol. 53(4):1007-19
  • Pereira-da-Silva M, Torsoni MA, Nourani HV, Augusto VD et al 2003. Hypothalamic melanin-concentrating hormone is induced by cold exposure and participates in the control of energy expenditure in rats. Endocrinology, vol(11):4831-40.