Dr Natasha Vassileff

PhD
Postdoctoral Fellow

I am an early career postdoctoral researcher investigating neurodegenerative diseases and neuroinflammation. My PhD at La Trobe University focused on understanding how extracellular vesicles (EVs), small particles involved in intercellular communication, play a role in propagating neurodegenerative diseases through alterations in protein and miRNA cargo. Throughout my PhD studies, I successfully isolated EVs from human post-mortem brain tissue samples, serum samples, and cell culture supernatants. I became well versed in miRNA next generation sequencing and analysis, digital and RT-PCR, and proteomics analysis including identification of post-translational modifications. Furthermore, through animal studies characterizing the role EVs play in facilitating ALS, I was able to become proficient in working with animal models. I recently joined the Clear Vision Research Group at the Australian National University where I will continue researching the role of neuroinflammation in neurodegenerative diseases. I hope to identify protein post translational modifications induced through neuroinflammation and their effect on the pathogenesis and progression of neurodegeneration.

Research interests

Neurodegeneration

Neuroinflammation

Post-translational modifications

Proteomics

  • Zhao, W, Spiers, J, Vassileff, N et al. 2023, 'microRNA-146a modulates behavioural activity, neuroinflammation, and oxidative stress in adult mice', Molecular and Cellular Neuroscience, vol. 124, pp. 1-11.
  • Spiers, J, Vassileff, N & Hill, A 2022, 'Neuroinflammatory Modulation of Extracellular Vesicle Biogenesis and Cargo Loading', NeuroMolecular Medicine, vol. 24, pp. 385â391.
  • Juliani, J, Vassileff, N & Spiers, J 2021, 'Inflammatory-Mediated Neuron-Glia Communication
    Modulates ALS Pathophysiology', Journal of Neuroscience, vol. 41, no. 6, pp. 1142-1144.
  • Vassileff, N, Vella, L, Rajapaksha, H et al. 2020, 'Revealing the Proteome of Motor Cortex Derived Extracellular Vesicles Isolated from Amyotrophic Lateral Sclerosis Human Postmortem Tissues', Cells, vol. 9, no. 7, pp. 1-20.
  • Vassileff, N, Cheng, L & Hill, A 2020, 'Extracellular vesicles � propagators of neuropathology and sources of potential biomarkers and therapeutics for neurodegenerative diseases', Journal of Cell Science, vol. 133, no. 23, pp. 1-15.