Single Cell, Cell Metabolism Analysis

Seahorse Xfe96 Extracellular Flux Analyzer

  • Measure the metabolism of cells, small tissue segments or isolated mitochondria in a 96 well format:

    • Oxygen consumption rate (OCR) to indicate mitochondrial respiration and
    • Extracellular Acidification Rate (ECAR) to measure the glycolytic pathway
  • Can inject drugs up to four times during the assay

For help with this instrument contact Mick Devoy.

Fluidigm C1

  • Isolates single cells into individual reaction chambers in an integrated fluidic circuit (IFC) in single-cell templates in preparation for mRNA sequencing, DNA sequencing, epigenetics or miRNA expression.
  • Cells can be automatically lysed and template prepared for qPCR or sequencing analysis, 4 hours.
  • Able to automatically stain captured cells for examination by microscopy for viability, surface markers or reporter genes.

For help with this technique contact peter.milburn@anu.edu.au.

Updated:  23 January 2018/Responsible Officer:  Director, JCSMR/Page Contact:  Web Manager