Microscopy

The ICF provides access to a range of light microscopy techniques including confocal, multiphoton, live cell and intra-vital imaging.

For inquiries please contact staff

Multiphoton

Olympus FVMPE-RS multi-photon microscope

  • Spectra Physics DeepSee Insight laser dual ouput : tunable 700 – 1300nm and 1040nm fixed line
  • 25x objective lens: water immersion, 1.05 NA, WD 2mm, correction collar 1-0.23, transmission 400 – 1600nm
  • 5x objective lens: M Plan Achromat, 0.1 NA, WD 20 mm
  • Prior motorised stage
  • Galvo and resonant scanner
  • 2 GaAsP non descanned detectors
  • 2 PMTs
  • Heating pads for mouse and rat

Confocal

Leica SP5 confocal microscope

  • Leica DMi6000 automated inverted microscope
  • Objectives lenses- 10x/0.3, 20x/0.7, 40x/0.8 W, and 63x/1.40 Oil, long working distance 40X,
  • Laser lines 405, 458, 476, 488, 514, 561, and 633nm
  • 5 channel spectral detection: 4 PMT detectors and one HyD high sensitivity, low noise detector
  • I transmission detector
  • Super Galvo Z stage
  • FRAP and FRET, deconvolution
  • Leica LAS AF software
  • Live cell chamber – temperature and CO2 control
  • Plate holder stage insert

Fluorescence and bright-field

Olympus IX71 inverted bright-field / fluorescence microscope

  • Objective lenses 4x, 10 x, 20 x, 40x, 60x oil, 100x oil,
  • DIC
  • Filters:
  • Ex 330-385 and 420 LP,
  • ex 460-495 and em 505LP,
  • ex 460-495 and em 510-55-BP,
  • ex 510-550 and em 590LP,
  • ex 545-589 and em 610.
  • Olympus DP70 11 megapixel colour camera

Olympus IX81 live cell, inverted brightfield/fluorescence microscope

  • Cell R multi-dimensional control software
  • Incubator enclosure, humidity and CO2 control
  • Objectives” lenses 4x, 10 x, 20 x, 40x, 60x oil, 100x oil
  • Filters for DAPI, FITC, CY3, TxRed and CFP/YFP

Zeiss Axio Observer with Apotome

Nikon TE3000 inverted fluorescence microscope with video camera and flow chamber

Coming soon

  • Objectives lenses: 4x, 10x, 20x, 40x, 100x oil
  • Filters: N B-2A (FITC, GFP, Alexa 488) and N G-2A (Alexa 546, 555, 594, Cy3, PI)
  • Video camera
  • Heating plate – Tokai Hit
  • Flow chamber- Glycotech
  • Live cell chamber – temperature and CO2 control

 

 

Zeiss Axiophot upright brightfield/fluorescence microscope

  • Filters for DAPI, FITC and CY3

 

Stereo fluorescence

 

Leica Stereo brightfield / fluorescence microscope

  • Filters: GFP, Texas Red, violet and UV
  • Leica DC500 camera

Updated:  23 January 2018/Responsible Officer:  Director, JCSMR/Page Contact:  Web Manager