Flow Cytometry

Cell Sorting

The FACS lab provides a cell sorting service for researchers and students. There are 2 cell sorters operated by a flow cytometry specialist and operator between 9 - 5pm week days with after-hours sorting by special arrangement.

Please contact Harpreet Vohra or Mick Devoy for any questions about flow cytometry, how to design experiments, prepare samples, or to analyse your data.

BD FACS Aria II

High speed cell sorter with 4 lasers

  • 488nm Blue green laser: 2 scatter and 5 fluorescent channels
  • 633nm Red laser: 3 fluorescent channels
  • 405nm Violet laser: 3 fluorescent channels
  • 561nm Yellow Green laser: 6 fluorescent channels

BD FACS Aria I

High speed cell sorter with 3 lasers

  • 488nm Blue green laser: 2 scatter and 5 fluorescent channels
  • 633nm Red laser: 3 fluorescent channels
  • 405nm Violet laser: 3 fluorescent channels

Analysers

Training is provided for users of these instruments. Access is 24/7 for experienced operators.

BD Fortessa X-20

Bench top analyser with 5 lasers

  • 488nm Blue green laser: 2 scatter and 2 fluorescent channels
  • 633nm Red laser: 3 fluorescent channels
  • 561nm Yellow/green: 5 fluorescent channels
  • 405nm Violet laser: 6 fluorescent channels
  • 350nm UV laser: 2 fluorescent channels

BD Fortessa

Bench top analyser with 5 lasers

  • 488nm Blue green laser: 2 scatter and 5 fluorescent channels
  • 633nm Red laser: 3 fluorescent channels
  • 561nm Yellow/green: 6 fluorescent channels
  • 405nm Violet laser: 4 fluorescent channels
  • 350nm UV laser: 2 fluorescent channels

BD LSRII cytometer (MCRF)

Bench top analyser with 3 lasers

  • 488nm Blue green laser: 2 scatter and 5 fluorescent channels
  • 633nm Red laser: 3 fluorescent channels
  • 405nm Violet laser: 3 fluorescent channels

BD LSRII cytometer (APF)

Bench top analyser with 3 lasers

  • 488nm Blue green laser: 2 scatter and 5 fluorescent channels
  • 633nm Red laser: 3 fluorescent channels
  • 405nm Violet laser: 2 fluorescent channels

BD FACS Calibur

Bench top analyser with 2 lasers

  • 488nm blue green laser: 2 scatter and 3 fluorescent parameters
  • 633nm red diode laser: 1 fluorescent channel

Please check with FACS lab staff for additional filter sets for these instruments.

Updated:  25 May 2018/Responsible Officer:  Director, JCSMR/Page Contact:  Web Manager