Bio-Optics Imaging Lab

An interdisciplinary bioimaging development lab set up by Dr Steve Lee from the Research School of Engineering (CECS) in collaboration with

JCSMR Imaging and Cytometry Facility and ARC Centre of Excellence Advanced Molecular Imaging.

Funded: FERL start up grant, ARC Early Career Award, ANU Major Equipment grant

Poly-Scan: High speed polygon scanning intravital microscope

Customized instrumentation

Software

Advanced Optics

Microendoscopy

High Speed Imaging

Technical Data

  1. Ti-Saphhire Laser (Spectra Physics) – 80MHz, 800mW, 690-920 nm
  2. Two PMT's for 2 color detection green and red or red and far red ranges
  3. 40 fps - 60 fps (512 X 512), 320- 480 fps (512X64)
  4. Multi-channel data acquisition and image acquisition system
  5. Objectives:  X40 (NA 0.7, Air), X40 (NA 0.8,. Air)
  6. Microendoscope: X40 (NA 0.8,. Air)
  7. Adaptive optics (Deformable mirror)
  8. Customised Flexible software interface for high speed imaging

 

         

ASI Light Sheet Microscopy coming soon

Technical Data

  • Field of View* >400 µm diagonal
  • Resolution* 380 nm @ 500 nm wavelength in XYZ Sample Size
  • Unlimited for flat samples, up to 3.5 mm radius hemisphere
  • Depends on objective, these are for Nikon 40x/0.8 WD. Mounting Cover slip or open dish Imaging
  • Depth Limited by scattering, usually 30 -150 µm depending on sample
  • Software:Various free/open-source and proprietary Photomanipulation Available using inverted microscope objective Incubation 25-40 °C with CO2 and humidity control (others possible)
  • Compatible Cameras: Any sCMOS with external trigger
  • Compatible Lasers: Any with TTL control (dual fiber output beneficial) Acquisition Modes Synchronized slice/piezo Stage scan Fixed sheet Multi-D Acquisition Any combination of: Time Points Multi-position Multi-color (up to 4)

Updated:  22 April 2018/Responsible Officer:  Director, JCSMR/Page Contact:  Web Manager