Messenger ribonucleic acids (mRNA) act as templates for protein synthesis by ribosomes. Much like the DNA of genes, eukaryotic mRNA molecules do not exist as naked nucleic acid. They interact with RNA-binding proteins, ribosomes and translation factors, and adopt dynamic structures that determine the precise outcome of translation. We combine NGS technology with isolation of polyribosomal complexes from living cells, to generate transcriptome-wide snapshots of the distribution of translation complexes along mRNAs. Such systems-level data will allow unprecedented insight into the mechanism, dynamics and regulation of protein synthesis.
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