Emerging Pathogens and Vaccines Program
Recent Publications

2009

Alsharifi M, Furuya Y, Bowden TR, Lobigs M, Koskinen A, Regner M, Trinidad L, Boyle DB, Müllbacher A (2009). Intranasal flu vaccine protective against seasonal and H5N1 avian influenza infections. PLOS ONE 4 (4): e5336

Lobigs M, Müllbacher A, Regner M (2009). CD4+ and CD8+ T cell immune responses in West Nile virus infection. In: Diamond M ed. West Nile encephalitis virus infection: viral pathogenesis and the host immune response. Springer Series: Emerging Infectious Diseases of the 21st Century. Springer, New York, pp 287-309.

Lobigs M, Larena M, Alsharifi M, Lee E, Pavy M (2009) Live chimeric and inactivated Japanese encephalitis virus vaccines differ in their cross-protective value against Murray Valley encephalitis virus. J. Virol. 83: 2436-2445.

Pardo J, Aguilo JI, Anel A, Martin P, Joeckel L, Borner C, Wallich R, Müllbacher A, Froelich CJ, Simon MM.The biology of cytotoxic cell granule exocytosis pathway: granzymes have evolved to induce cell death and inflammation. Microbes Infect. 2009 Apr;11(4):452-9. Epub 2009 Feb 26. Review.

Ranasinghe C, Ramshaw IA. Immunisation Route Dependent Expression of IL-4/IL-13 Can Modulate HIV-Specific CD8+ CTL Avidity., 2009 Euro J
Immunol, 39 (7), 1819-30.

Ranasinghe C, Ramshaw IA. Genetic heterologous prime-boost vaccination strategies for improved systemic and mucosal immunity., 2009 Expert Rev. Vaccines (In press).

Regner M, Pavlinovic L, Koskinen, A, Young N, Trapani J, Müllbacher A. (2009) Cutting edge: Rapid and efficient in vivo cytotoxicity by cytotoxic T cells is independent of granzyme A and B. 183(1):37-40. Epub 2009 Jun 12.

2008

Alsharifi, M., Lobigs, M., Bettadapura, J., Koskinen, A., and Mullbacher, A., 2008, Restricted Semliki Forest virus replication in communication perforin and fas-ligand double-deficient mice. Journal of General Virology 89, 1942-1944 Part 8.

Alsharifi, M., Mullbacher, A., and Regner, M., 2008, Interferon type I responses in primary and secondary infections. Immunology and Cell Biology 86(3), 239-245.

Chung, WY, Sack, M, Carter, R, Spiegel, H, Fischer, R, Hirst, TR, Williams, NA, and James, RFL (2008) Phage-display derived single-chain fragment variable (scFv) antibodies recognizing conformational epitopes of Escherichia coli heat-labile enterotoxin B-subunit. Journal of immunological Methods 339(2): 115-123.

Day, S.L., Ramshaw, I.A., Ramsay, A.J., and Ranasinghe, C., 2008, Differential effects of the type I interferons alpha4, beta, and epsilon on antiviral activity and vaccine efficacy. Journal of Immunology 180(11), 7158-7166.

Lee, E. and Lobigs, M., 2008, E protein domain III determinants of yellow fever virus 17D vaccine strain enhance binding to glycosaminoglycans, impede virus spread, and attenuate virulence. Journal of Virology 82(12), 6024-6033.

Lobigs M, Müllbacher A, Regner M (2008). CD4+ and CD8+ T cell responses in West Nile virus infection. In “West Nile encephalitis virus infection: Viral pathogenesis and the host immune response” (Michael Diamond, ed.)

Pardo J, Simon MM, Regner M, Anel A. Immunological Cytotoxic Factors. In: ENCYCLOPEDIA OF LIFE SCIENCES (John Wiley & Sons Ltd); posted online Dec 15, 2008

Pardo, J., Wallich, R., Martin, P., Urban, C., Rongvaux, A., Flavell, R.A., Mullbacher, A., Borner, C., and Simon, M.M., 2008, Granzyme B-induced cell death exerted by ex vivo CTL: discriminating requirements for cell death and some of its signs. Cell Death and Differentiation 15(3), 567-569.

Regner, M., Fenner, F., and Mullbacher, A., 2008, Mousepox and Rabbitpox Viruses. 3rd ed. Encyclopedia of Virology, eds. B.W.J. Mahy and M.H.V. van Regenmortel. 3. pp. 342-348.

2007

Budd A, Alleva L, Alsharifi M, Koskinen A, Smythe V, Mullbacher A, Wood J, Clark I. (2007). Increased Survival after Gemfibrozil Treatment of Severe Mouse Influenza. Antimicrobial Agents and Chemotherapy, 51 (8), 2965-2968.

de Jersey, J., Snelgrove, S.L., Palmer, S.E., Teteris, S.A., Mullbacher, A., Miller, J.F.A.P., and Slattery, R.M., 2007, {beta} Cells cannot directly prime diabetogenic CD8 T cells in nonobese diabetic mice. Proceedings of the National Academy of Sciences of the United States of America 104(4), 1295-1300.

De Rose, R., Batten, C.J., Smith, M.Z., Fernandez, C.S., Peut, V., Thomson, S., Ramshaw, I.A., Coupar, B.E.H., Boyle, D.B., Venturi, V., Davenport, M.P., and Kent, S.J., 2007, Comparative efficacy of subtype AE simian-human immunodeficiency virus priming and boosting vaccines in pigtail macaques. Journal of Virology 81(1), 292-300.

Pardo J, Wallich R, Ebnet K, Iden S, Zentgraf H, Martin P, Ekiciler A, Prins A, Müllbacher A, Huber M and Simon M M. (2007). Granzyme B is expressed in mouse mast cells in vivo and in vitro and causes delayed cell death independent of perforin. Cell Death and Differentiation 14, 1768-1779.

Sugui JA, Pardo J, Chang YC, Mullbacher A, Zarember KA, Galvez EM, Brinster L, Zerfas P, Gallin JI, Simon MM, Kwon-Chung KJ.(2007). Role of laeA in the regulation of alb1, gliP, conidial morphology and virulence in Aspergillus fumigatus.. Eukaryotic Cell. 6 (9), 1552-1561.

Sugui JA, Pardo J, Chang YC, Zarember KA, Nardone G, Galvez EM, Mullbacher A, Gallin JI, Simon MM, Kwon-Chung KJ. (2007). Gliotoxin is a virulence factor of Aspergillus fumigatus: gliP deletion attenuates virulence in mice immunosuppressed with hydrocortisone. Eukaryotic Cell. 6 (9), 1562-1569.

Sutton VR, Waterhouse NJ, Browne KA, Sedelies K, Ciccone A, Anthony D, Koskinen A, Mullbacher A and Trapani JA. (2007). Residual active granzyme B in cathepsin C-null lymphocytes is succicient for perforin-dependent target cell apoptosis. The Journal of Cell Biology, 176 (4) 425-433.

Ranasinghe, C., Turner, S.J., McArthur, C., Sutherland, D.B., Kim, J.H., Doherty, P.C., and Ramshaw, I.A., 2007, Mucosal HIV-1 pox virus prime-boost immunization induces high-avidity CD8(+) T cells with regime-dependent cytokine/granzyme B profiles. Journal of Immunology 178(4), 2370-2379.

Rollman, E., Smith, M.Z., Brooks, A.G., Purcell, D.F.J., Zuber, B., Ramshaw, I.A., and Kent, S.J., 2007, Killing kinetics of simian immunodeficiency virus-specific CD8(+) T cells: implications for HIV vaccine strategies. Journal of Immunology 179(7), 4571-4579.

2006

Alsharifi, M., Lobigs, M., Lee, E., Simon, M.M., Kersten, A., Müller, K., Koskinen, A. and Müllbacher, A. 2006. NK cell mediated immunopathology during an acute viral infection of the CNS. Eur. J. Immunol. 36(4): 887-896.

Alsharifi, M., Regner, M., Lobigs, M., Koskinen, A., Lee, E. and Müllbacher, A. 2006. Type-I Interferon exhaustion after a viral infection causes a transient period of reduced resistance to secondary infections. J.Immunol. 177(5), 3235-3241.

Coupar, B.E.H., Purcell, D.F.J., Thomson, S.A., Ramshaw, I.A., Kent, S.J., and Boyle, D.B., 2006, Fowlpox virus vaccines for HIV and SHIV clinical and pre-clinical trials. Vaccine 24(99), 1378-1388.

Dale, C.J., Thomson, S., De Rose, R., Ranasinghe, C., Medveczky, C.J., Pamungkas, J., Boyle, D.B., Ramshaw, I.A., and Kent, S.J., 2006, Prime-Boost Strategies in DNA Vaccines. DNA Vaccines - Methods and Protocols, eds. W.M. Saltzman, Totowa, New Jersey, USA: Humana Press Inc. pp. 171-198.

De Rose, R., Sullivan, M.T., Dale, C.J., Kelleher, A.D., Emery, S., Cooper, D.A., Ramshaw, I.A., Boyle, D., and Kent, S.J., 2006, Dose-response relationship of DNA and recombinant fowlpox virus prime-boost HIV vaccines: Implications for future trials. Human Vaccines 2(134-136).

Halenius A, Momburg F, Reinhard H, Bauer D, Lobigs M, Hengel H (2006). Physical and functional interactions of the cytomegalovirus US6 glycoprotein with the transporter associated with antigen processing (TAP). J. Biol. Chem. 281: 5383-5390.

Harrison, J.M., Bertram, E.M., Boyle, D.B., Coupar, B.E.H., Ranasinghe, C., and Ramshaw, I., 2006, 4-1BBL coexpression enhances HIV-specific CD8 T cell memory in a poxvirus prime-boost vaccine. Vaccine. 24(47-48), 6867-6874.

Harrison, J.M. and Ramshaw, I.A., 2006, Cytokines, skin, and smallpox - A new link to an antimicrobial peptide. Immunity 24(3), 245-247.

Hirst TR and D’Souza JM (2006) Escherichia coli enterotoxin B subunit as a potent immunomodulator and peptide delivery vehicle. In “Recent Research Developments on Bacterial & Plant Toxins”. L Johannes and D Gillet (eds) (in press). Review.

Hirst TR and D’Souza JM (2006) Vibrio cholerae and Escherichia coli thermolabile enterotoxin. In “A Comprehensive Sourcebook of Bacterial Protein Toxins” 3rd Edition J E Alouf and M Popoff (eds) Academic Press pp 270-90 Review.

Lee E, Pavy M, Young N, Freeman C, Lobigs M (2006). Antiviral effect of the heparan sulfate mimetic, PI-88, against dengue and encephalitic flaviviruses. Antiviral Res. 69: 31-38.

Lee E, Wright PJ, Davidson A, Lobigs M (2006). Virulence attenuation of dengue virus due to augmented glycosaminoglycan-binding affinity and restriction in extraneural dissemination. Journal of General Virology 87, 2791-2801. Accessible on-line in JGV Direct, a service for rapid publication of papers considered by the Editors to be of special importance.

Liu WJ, Wang XJ, Clark DC, Lobigs M, Hall RA, Khromykh AA (2006). A single amino acid substitution in the West Nile virus nonstructural proteinNS2A disables its ability to inhibit interferon alpha/beta induction and attenuates virus virulence in mice. Journal of Virology 80, 2396-2404.

May F, Lobigs M, Lee E, Gendle, Mackenzie JS, Broom AK, Conlan JV, Hall RA (2006). Biological, antigenic and phylogenetic characterisation of the flavivirus Alfuy. J Gen. Virol. 87: 329-337.

May, F., Lobigs, M., Lee, E., Gendle, Mackenzie, J.S., Broom, A.K., Conlan, J.V., and Hall, R.A., 2006, Biological, antigenic and phylogenetic characterisation of the flavivirus Alfuy. Journal of Gen Virology 87, 329-337.

Müllbacher, A., Lobigs, M., Alsharifi, M. and Regner, M. 2006. Cytotoxic T cell immunity as target for influenza vaccines. The Lancet Infectious Disease 6:255-256.

Pardo, J., Urban, C., Galvez, E. M., Ekert, P. G., Müller U., Kwon-Chung, J., Lobigs, M., Müllbacher, A., Wallich, R., Borner, C. and Simon, M. M.(2006) The mitochondrial protein Bak is pivotal for gliotoxin-induced apoptosis and a critical host factor of A. fumigatus virulence in mice. J. Cell Biol. 174: 509-519.

Ranasinghe, C., Medveczky, J.C., Woltring, D., Gao, K., Thomson, S., Coupar, B.E.H., Boyle, D.B., Ramsay, A.J., and Ramshaw, I.A., 2006, Evaluation of Fowlpox-Vaccinia Virus Prime-Boost Vaccine Strategies for High-Level Mucosal and Systemic Immunity Against HIV-1. Vaccine.24(31-32), 5881-5895.

Röckendorf N, Bade S, Hirst TR, Gorris, H-H, Frey A (2006) Synthesis and Functional Characterization of a Fluorescent Ganglioside GM1 Derivative and Screening of a Synthetic Peptide Library for GM1 Binding Sequence Motifs Bioconjug Chem 18(2):573-8.

Rolph, M., S.,, Young, T., R.,, Shum, B.O.V., Gorgun, C.Z., Schmitz-Peiffer, C., Ramshaw, I.A., Hotamisligil, G.S., and Mackay, C.R., 2006, Regulation of dendritic cell function and T cell priming by the fatty acid-bindilng protein aP2. Journal of Immunology 177(11), 7794-7801.

Wang, Y., Lobigs, M., Lee, E., Koskinen, A., and Müllbacher, A. 2006 CD8+ T cell mediated recovery and or immunopathology in WNV encephalitis is independent of interferon-gamma.Journal of General Virology 87, 3599-3609.


2005

Alsharifi, M., Regner, M., Lobigs, M., Koskinen, A., Lee, E. and Müllbacher, A. 2005. Type-I interferons, induced in response to virus infections, trigger systemic, partial, lymphocyte activation. J Immunol. 175: 4635-4640.

Chea, S., Dale, C.J., De Rose, R., Ramshaw, I.A., and Kent, S.J., 2005, Enhanced cellular immunity in macaques following a novel peptide immunotherapy. Journal of Virology 79(6), 3748-3757.

Dale, C. J., Thomson, S., De Rose, R., Ranasinghe, C., Medveczky, C. J., Pamungkas, J., Boyle, D. B., Ramshaw, I. A., Kent, S. J.,
Prime-Boost Strategies in DNA Vaccines - Methods in Molecular Medicine, Vaccine Methods and protocols 2nd edition, edited by W.M.Saltzman, Human press, New Jersey

De Rose, R., Chea, S., Dale, C.J., Reece, J., Fernandez, C.S., Wilson, K.M., Thomson, S., Ramshaw, I.A., Coupar, B.E.H., Boyle, D.B., Sullivan, M.T., and Kent, S.J., 2005, Subtype AE HIV-1 DNA and recombinant Fowlpoxvirus vaccines encoding five shared HIV-1 genes: safety and T cell immunogenicity in macaques. Vaccine 23(16), 1949-1956.

Doan, T., Herd, K., Ramshaw, I., Thomson, S., and Tindle, R.W., 2005, A polytope DNA vaccine elicits multiple effector and memory CTL responses and protects against human papillomavirus 16 E7-expressing tumour. Cancer Immunology Immunotherapy 54(2), 157-171.

Hu, K.F., Regner, M., Siegrist, C.A., Lambert, P.H., Chen, M., Lövgren Bengtsson, K., , and Morein, B., (2005). The immunomodulating properties of HRSV ISCOMs containing Quillaja saponin components QH-A, QH-C and ISCOPREP-703. FEMS Immunol. Med. Mic. 43:2, 269-276.

Kent, S.J., Dale, C.J., Ranasinghe, C., Stratov, I., De Rose, R., Chea, S., Montefiori, D.C., Thomson, S., Ramshaw, I.A., Coupar, B.E., Boyle, D.B., Law, M., Wilson, K.M., and Ramsay, A.J., 2005, Mucosally-administered human-simian immunodeficiency virus DNA and fowlpoxvirus-based recombinant vaccines reduce acute phase viral replication in macaques following vaginal challenge with CCR5-tropic SHIV(SF162P3). Vaccine 23(42), 5009-5021..

Lobigs M, Lee E (2005). Coordination of cleavages at the flavivirus C-prM junction: role in virus assembly and virulence. In: Ryan PD, Aaskov JG, St George TG, Dale PER eds. Arbovirus Res. Aust. 9: pp 202-206.

Martin, P., Wallich, R., Pardo, J., Müllbacher, A., Munder, M., Modolell, M. and Markus M. Simon. 2005. Mouse granulocytes do not express granzyme A, granzyme B and perforin, independent of their activation state: similarities or differences with human polymorphonuclear leukocytes? Blood 106:2871-2878.

May FJ, Lee E, Lobigs M, Hall RA (2005). Genetic, antigenic and biological characterization of Alfuy virus. In: Ryan PD, Aaskov JG, St George TG, Dale PER eds. Arbovirus Res. Aust. 9: pp 207-210 .

Paccani SR, Boncristiano M, Patrussi L, Ulivieri C, Wack A, Valensin S, Hirst TR, Amedei A, Del Prete G, Telford JL, D'Elios MM, Baldari CT. (2005) Defective Vav expression and impaired F-actin reorganization in a subset of patients with common variable immunodeficiency characterized by T-cell defects. Blood 106(2):626-34.

Pamungkas, J., De Rose, R., Iskandriati, D., Noviana, R., Paramastri, Y., Dale, C.J., Shoobridge, M., Medveczky, C.J., Ramshaw, I.A., Thomson, S., and Kent, S.J., 2005, Comparison of whole gene and whole virus scrambled antigen approaches for DNA prime and fowlpox virus boost HIV type 1 vaccine regimens in macaques. AIDS Research and Human Retroviruses 21(4), 292-300.

Pao, L. I., Sumaria, N., Kelly, J. M., Cretney, E., Wallace, M. E., Uldrich, A., Godfrey, D. I., Papadimitriou, J. M., Müllbacher, A., Rajewski, K., Degli-Eposti, M. A. and Smyth, M.J. 2005. The NK cell protease granzyme M is not critical for NK cell-mediated death, but is involved in host innate response to cytomegalovirus infection. J. Immunol. 175: 3235-3243.

Robbins, S.J., Jackson, R.J., Fenner, F., Beaton, S., Medveczky, J., Ramshaw, I.A., and Ramsay, A.J., 2005, The efficacy of cidofovir treatment of mice infected with ectromelia (mousepox) virus encoding interleukin-4. Antiviral Research 66(1), 1-7.
Thomson, S.A., Jaramillo, A.B., Shoobridge, M., Dunstan, K.J., Everett, B., Ranasinghe, C., Kent, S.J., Gao, K., Medveckzy, J., Ffrench, R.A., and Ramshaw, I.A., 2005, Development of a synthetic consensus sequence scrambled antigen HIV-1 vaccine designed for global use. Vaccine 23(38), 4647-4657.