Gene Expression from single cells, Pathway analysis and digital PCR on the QuantStudioTM 12K Flex (available at the ACRF Biomolecular Resource Facility).
Presented by Yash Tiwari PhD – Field Application Scientist.
Seminar Outline: When should I use High Density qPCR? What are the benefits of High Density qCPR with the OpenArray/TaqMan Array Cards? What are the main steps (QC steps, workflow, data analysis …)? What are the applications:
- Pathway Analysis – screen/profile entire pathways quickly & easily with the OpenArray Panels and Taqman array cards
- miRNA profiling – from as little as 1 ng
- Single cell analysis – mRNA and miRNA analysis from a single cell (on your current instrument)
What are the next steps – what to do after my high density qPCR experiments (biomarkers discovery, validation …)? Q&A
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