Associate Professor Katharina Gaus, Cellular Membrane Biology Laboratory, Centre for Vascular Research, Faculty of Medicine, University of New South Wales.
Professor Katharina Gaus is an NHMRC Senior Research Fellow and leads the Cell Membrane Biology group at the Centre for Vascular Research, University of New South Wales. The main objective of her research is to identify the principles that govern the organization of the plasma membrane, and thus define the role of membrane domains in signal transduction processes. Her team’s contribution is to use novel fluorescence microscopy approaches to define the molecular mechanisms that regulate the temporal and spatial organization of cell signalling.
T cell activation begins with the formation of signaling complexes at the cell surface involving the T cell antigen receptor (TCR), the Src family kinase Lck and the adaptor protein, linker for activation of T cells (LAT). We have established single molecule fluorescence imaging approaches known as localization microscopy to determine how TCR engagement reorganizes the T cell membrane on the molecular scale. I will present two examples of how single-molecule imaging in intact cells provides insights into the regulation of T cell signaling that could not have been obtained by any other means.