Dr Simon Hunt, FHEA - Emeritus Lecturer in Immunology, Sir William Dunn School of Pathology, Oxford University, UK
The dynamics of calcium signalling within recently-triggered B or T lymphocytes is best measured by video-imaging single cells labelled with calcium-sensitive fluorescent indicators like Fluo-4. Individual cells display widely differing calcium dynamics, even when stimulated identically. We developed a cell population array imaging instrument to measure whole-cell calcium fluctuations in up to 10000 cells at once over periods of tens of minutes, each stably confined in its own ultramicrowell. We record signals from 100-fold more cells than by conventional video microscopy. With such data acquisition rates, we're exploring how far we may identify lymphocyte subpopulations not by molecular phenotype but according to their signal shapes (e.g. oscillators; peak-transients; peak-plateau, etc). Could array imaging be useful to predict gene expression decisions regulated by calcium in lymphocyte subsets, and to investigate intracellular calcium regulation?
Mini-bio at www.keble.ox.ac.uk/academics/about/dr-simon-v-hunt Cell population array imaging: concept and instrument at www.cpai.eu