JCSMR Flow Cytometry (FACS Lab.)

Texts Page

Last updated : 18 Feb, 1998

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Index of Texts available in the lab.

1. Methods Texts
2. Reference Texts
3. Some of the Catalogues (commercial) available for loan
4. Flow Cytometry Course manuals

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Texts containing Methods and Protocols

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"Handbook of Flow Cytometry Methods."

J.Paul Robinson, Editor

Associate Editors:

Zbigniew Darzynkiewicz,
Phillip Dean,Lynn Dressler,
Hans Tanke, and Leon Wheeless.

Major Contents headings of this text are:

Standards, Reagents, & Instruments

Precautions

Methods

• Phenotypic Analysis
  
• Measurement of DNA
  
• Other General Flow Methods
  
• Methods for Functional Assessment
  
• Associated Methods
  

Data Analysis & Computers

Lab Comments:Handbook of Flow Cytometry
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"Flow Cytometry: A Practical Approach."

M.G.Ormerod, Editor

Major Contents headings of this text are:

1. Introduction to the principles of flow cytometry

2. An introduction to fluorescence technology

3. Measurement of cellular subsets using antibodies

4. Analysis of DNA

• 4A. General Methods
• 4B. Measurement of tumour cell kinetics by Bromodeoxyuridine method.
• 4C. Cell kinetic analysis using continuous BrdU labelling and bivariate Hoechst 33358/Ethidium Bromide flow cytometry.

5. Cell Sorting

• 5A. Using the Coulter Epics C
• 5B. Using the Becton Dickinson FACS for sorting
• 5C. On the Ortho Cytofluorograf

6. Chromosome analysis and sorting

7. Intracellular ionized calcium membrane

8. Flow cytometry of RNA content

9. Nuclear associated antigens

10. Analysis of radiation and chemical damage in mammalian cells

11. Measurement of dynamic cellular events

12. Further applications to cell biology

Lab Comments:Flow Cytometry: A Practical Approach
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List of some of the Commercial catalogues available in the lab.

These catalogues are available for loan, with some providing extensive information and references. THESE CATALOGUES ARE IN NO WAY RECOMMENDATIONS OF A PARTICULAR COMPANY OR A PARTICULAR COMPANIES PRODUCTS.

Becton Dickinson Reagent Catalog

Bioclone Product Catalogue

Coulter Cytometry Catalog

Caltag Laboratories: Catalog 1995-1996

Dako 1995 Catalog

Endogen Inc. Product Reference Guide

Pharmingen Research Products Catalog 1994-95

R&D Systems 1995 Catalog

Serotec 1995/96 Product Guide

Zymed Laboratories Inc. Immunochemical Products 1994-1995 Catalog

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Flow Cytometry Course Manuals

There are a number of course manuals available in the laboratory. These manuals contain a wealth of methods, in different aspects of flow.

The latest addition to these manuals is the manual from the National Course in Flow Cytometry, Los Alamos, New Mexico, USA. This looseleaf book is some 12 cm. thick and even covers such areas as "How to build a flow cytometer". For more detailed information on the contents of these manuals, drop pass, drop us an email, or phone.

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The creator of this page:Geoff.Osborne@anu.edu.au

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