Equipment
LSR II
For more
information about the LSR II click here.
For more information about the HTS
attachment click here
To download a step by step manual
to run plates on the HTS click here
or download the Daily Operation Tutorial
The
BD - LSR Benchtop Flow Cytometer
Click on the image for more information about the LSR.
Vantage DiVa:
More information
coming soon!
FACStar plus:
The
FACStar plus is equipped with a triple laser bench, Macrosort Sort Option
(for large particles), Sort Enhancement Module (allows nonrectangular sort
windows), Pulse Processor (Ratio Measurements for Calcium Studies, Doublet
Discrimination for DNA Analysis) and an Automatic Cell Deposition Unit.
The ACDU is used to sort one or more cells per well into plates from Terasaki to 24 well plates or slides.
There is a cooling/heating unit attached which allows temperature control from 4°C - 56°C.
Laser 1 is a Coherent Argon-Ion water cooled laser tuned to 488nm wavelength.
Laser 2 is a Spectra Physics Argon-Ion water cooled laser tuned to UV.
Laser 3 is a Helium-Neon air cooled laser at 632nm wavelength.
BD FACSCalibur:
The FACSCalibur is an analytical
flow cytometer with two scattered light detectors and four fluorescence detectors.
The fourth fluorescence detector option which this instrument has collects
signals from fluorochromes excited by a small 633nm red diode laser.
Choose this cytometer in preference to the BD LSR, if you are working with APC and a tandem dye such as PE-CY5 as in practice you will have less difficulty with the compensation for spectral overlap on this instrument.
The FACSCalibur is also fitted with the Multiwell Acquistion System with allows samples to be taken directly from 96 and 384 well plates
If you'd like to use this
unit, please read the MAS User Guide PDF file and remember
to remove the MAS probe .
FACSort:
The
FACSort can measure up to six parameters. FSC, SSC and four fluorescence parameters.
It can also measure the pulse height, area and width of three fluoresence
parameters (FL1, FL2 and FL3).
Some of the most common fluoresent dye's used on FACSort here at the JCSMR.
| Fluorescent Dye | Filter | Peak Emmision | Detector |
| FITC ( Fluorescin Isothiocyanate) | 530nm/30 | 525nm | FL1 |
| Phycoerythrin | 585nm/42 | 578nm | FL2 |
| Propidium Iodide | 585nm/42 or 670LP | 617nm | FL2 / FL3 |
| PerCp (Peridinin chlorphyll protein) |
670LP | 675nm | FL3 |
| Cychrome/Tri-colour (PE-Cy5) | 670LP | 670nm | FL3 |
| APC | 661nm/16 | 660nm | FL4 |
| Alexa 647 | 661nm/16 | 668nm | FL4 |
The flow rates on the FACSort are: LO ( 12µl ±3µl /min ) MED (35µl ±5µl /min) HI (60µl ±7µl/min)
FACScan:
 |
The FACScan is a single air cooled laser (488nm) instruments and are capable of three colour and two scatter parameter analysis. The FACScan is also equipped with a Doublet Discrimination Module for the measurement of pulse width and area which is used in DNA analysis.
Some of the most common dye's used on FACScan's here at the JCSMR.
| Fluorescent Dye | Peak Emmision | Detector |
| FITC ( Fluorescin Isothiocyanate) | 525nm | FL1 |
| Phycoerythrin | 578nm | FL2 |
| Propidium Iodide | 617nm | FL2 / FL3 |
| PerCp (Peridinin chlorphyll protein) |
675nm | FL3 |
| Cychrome/Tri-colour (PE-Cy5) | 670nm | FL3 |
For more information about fluorescence spectra's go to Joe Trotters Spectrum Viewer (Java) at the Scripps Research Institute.
Updated last 1-8-2005 by Sabine Grüninger